Ernie Yulyaningsih, Ivan A. Rudenko, Martin Valdearcos, Christian Vaisse, Suneil K. Koliwad, Allison W. Xu. Acute Lesioning and Rapid Repair of Hypothalamic Neurons outside the Blood-Brain Barrier. Cell Reports 19, 2257–2271 June 13, 2017 ª 2017 The Authors. http://dx.doi.org/10.1016/j.celrep.2017.05.060.

... goat anti-AgRP (1:1,000 Neuromics)...

Eun Ran Kim, Pei San Lew, Alexandra Spirkina, Tooru M. Mizuno. Xenin-induced feeding suppression is not mediated through the activation of central extracellular signal-regulated kinase signaling in mice. Behavioural Brain Research. Available online 15 June 2016. doi:10.1016/j.bbr.2016.06.026

...For AGRP immunofluorescence, sections were incubated with goat anti-AGRP (15 μg/ml, GT15023, Neuromics, Northfield, MN ...

Miyuki Shibata, Ryoichi Banno,Mariko Sugiyama,Takashi Tominaga,Takeshi Onoue,Taku Tsunekawa,1 Yoshinori Azuma, Daisuke Hagiwara,Wenjun Lu, Yoshihiro Ito, Motomitsu Goto, Hidetaka Suga,Yoshihisa Sugimura,Yutaka Oiso,and Hiroshi Arima.AgRP Neuron-Specific Deletion of Glucocorticoid Receptor Leads to Increased Energy Expenditure and Decreased Body Weight in Female Mice on a High Fat Diet. Endocrinology. Received May 16, 2015. Accepted February 16, 2016. ISSN Print 0013-7227 ISSN Online 1945-7170 doi: 10.1210/en.2015-1430 

...Immunohistochemistry in R26GRR reporter mice was performed as described previously (18). The antibody of tdsRed was purchased from Clontech (Palo Alto, CA). After washing, the sections were incubated for 1 hour at room temperature with the secondary antibodies (Alexa Fluor; Life Technologies, Carlsbad,CA). All fluorescently stained sections were examined with a fluorescence microscope (BZ-9000; Keyence, Osaka, Japan).AgRP and GR double immunohistochemistry was performed in female mice at the age of 16 weeks fasted for 48 hours as reported previously (18). The antibodies of AgRP and GR were purchased from NEUROMICS (Edina, MN) and S Assay Biotechnology (San Francisco, CA), respectively. After washing, the sections were incubated for 1 hour at room temperature with the secondary antibodies (Alexa Fluor; Life Technologies, Carlsbad,CA) and DAPI (Dojindo, Kumamoto, Japan). All fluorescently stained sections were examined with a confocal laser microscope (TiEA1R; NIKON INSTECH) and viewed using NIS-Elements software. The best-matched slice at 1.46 mm caudal from the bregma, according to the brain atlas (21), was chosen in each mouse for the analysis...

J. Nicholas Betley, Shengjin Xu, Zhen Fang Huang Cao, Rong Gong, Christopher J. Magnus, Yang Yu & Scott M. Sternson. Neurons for hunger and thirst transmit a negative-valence teaching signal. Nature 521, 180–185 (14 May 2015) doi:10.1038/nature14416

...goat anti-AGRP (1:5,000, Neuromics, GT15023)...

Alastair S Garfield, Chia Li, Joseph C Madara, Bhavik P Shah, Emily Webber,Jennifer S Steger, John N Campbell, Oksana Gavrilova, Charlotte E Lee, David P Olson, Joel K Elmquist, Bakhos A Tannous, Michael J Krashes & Bradford B Lowell. A neural basis for melanocortin-4 receptor–regulated appetite. Nature Neuroscience 18, 863–871 (2015) doi:10.1038/nn.4011

...AgRP Antibody (Neuromics GT15023, 1:1,000)...

J. Nicholas Betley, Shengjin Xu, Zhen Fang Huang Cao, Rong Gong, Christopher J. Magnus, Yang Yu & Scott M. Sternson. Neurons for hunger and thirst transmit a negative-valence teaching signal. Nature (2015) doi:10.1038/nature14416 Received 14 July 2014 Accepted 19 March 2015 Published online 27 April 2015

...goat anti-AGRP (1:5,000, Neuromics, GT15023)...

Cyprian Weavera, Nolan Turnerb, Jennifer Hal. Review of the Neuroanatomic Landscape Implicated in Glucose Sensing and Regulation of Nutrient Signaling: Immunophenotypic Localization of Diabetes Gene Tcf7l2 in the Developing Murine Brain. Journal of Chemical Neuroanatomy. Journal of Chemical Neuroanatomy. http://dx.doi.org/10.1016/j.jchemneu.2012.06.002

...Agouti-Related Protein(AgRP), Neuromics, GT15023, goat polyclonal, 5ug/ml...

Ai-Jun Li, Michael Foster Wiater, Marjolein T. Oostrom, Bethany R. Smith, Qing Wang, Thu T Dinh, Brandon L. Roberts, Heiko T Jansen, and Sue Ritter. Leptin-sensitive neurons in the arcuate nuclei contribute to endogenous feeding rhythms. Published online before print April 4, 2012, doi: 10.​1152/​ajpregu.​00086.​2012.

... goat anti-AGRP (1:1000;Neuromics)...

Yongheng Cao1, Masanori Nakata, Shiki Okamoto, Eisuke Takano, Toshihiko Yada, Yasuhiko Minokoshi, Yukio Hirata, Kazunori Nakajima, Kristy Iskandar, Yoshitake Hayashi, Wataru Ogawa, Gregory S. Barsh, Hiroshi Hosoda, Kenji Kangawa, Hiroshi Itoh, Tetsuo Noda, Masato Kasuga, Jun Nakae.PDK1-Foxo1 in Agouti-Related Peptide Neurons Regulates Energy Homeostasis by Modulating Food Intake and Energy Expenditure. PLoS ONE 6(4): e18324. doi:10.1371/journal.pone.0018324.

...For immunofluorescence analyses, mice were transcardially perfused with saline followed by 4% paraformaldehyde in 0.1 M phosphate-buffered saline, pH 7.4 (PBS). The brains were dissected and immersed in 4% paraformaldehyde at 4°C overnight and then soaked in 30% sucrose overnight. Frozen, free-floating coronal sections (4 µm thick) were cut through the arcuate nucleus with a microtome (Leica Microsystems). The sections were washed extensively in PBS for 20 min to quench endogenous peroxidase activity. For double staining of PDK1 and AGRP, the sections were stained with a Renaissance Tyramide Signal Amplification kit (#NEL701, Perkin Elmer, Waltham, MA) according to the manufacturer's protocol. The primary antibodies were Ab-241 (Signalway Antibody, Pearland, TX) for PDK1 and GT15023 (Neuromics, Edina, MN) for AGRP; the secondary antibodies were Alexa FluorR 594 chicken anti-rabbit IgG and Alexa FluorR 488 donkey anti-goat IgG (Molecular Probes, Eugene, OR). For double staining of Foxo1 and AGRP, we used an anti-FOXO1A antibody (ab12161, abcamR, Cambridge, UK), respectively. For double staining of FLAG and AGRP, the sections were stained using a Renaissance Tyramide Signal Amplification kit according to the manufacturer's protocol. The primary antibodies were the OctA-Probe (D-8: sc-807, Santa Cruz Biotechnology, Inc, Santa Cruz, CA); the secondary antibodies were Alexa FluorR 594 chicken anti-rabbit IgG and Alexa FluorR 488 donkey anti-goat IgG (Invitrogen, Carlsbad, CA)...

Glia Volume 52, Issue 3 , Pages 228 - 233 Published Online: 20 Jun 2005. Copyright © 2005 Wiley-Liss, Inc.
 

...goat anti-agouti-related protein (AgRP,. 15ug/ml, Neuromics, Bloomington, MN)...

Peter Kobelt, Anna-Sophia Wisser, Andreas Stengel, Miriam Goebel, Tobias Inhoff, Steffen Noetzel, R¨udiger W. Veh, Norbert Bannert, Ivo van der Voort, Bertram Wiedenmann, Burghard F. Klapp, Yvette Tache, Hubert Monnikes. Peripheral injection of ghrelin induces Fos expression in the dorsomedial hypothalamic nucleus in rats doi: 10.1016/j.brainres.2008.01.054

...AgRP; Neuromics, Minnesota, USA; 1:500 in a solution of 5% w/v donkey ....