Autofluorescence Blocking Agent: FluoMute
Autofluorescence Blocking Agent
Autofluorescence is a major obstacle to using immunofluorescence detection in cells and tissues. There are two major sources of autofluorescence:
- Native - Caused by such substances as porphyrins, flavins, and lipofuscins
- Fixation-induced - Caused by formation of fluorescent substances as a result of interaction between aldehyde fixatives with amines
Autofluorescence, regardless of origin, will obscure specific fluorescent signal when using primary and secondary antibodies conjugated to fluorescent probes.
FluoMute ready-to-use reagent reduces autofluorescence in cells and tissues, that is simple to add to your immunofluorescence ICC and/or IHC protocols. Treatment with FluoMute does not affect cell morphology and integrity of tissue antigens to be detected with primary antibodies. It is compatible with paraffin-embedded and frozen tissue sections, stem cells, lymphocytes and mammalian cells lines of different origin.
|FluoMute||SF40005||Blocking Reagent||Fluoro-Imaging||100 ml||$89|