Human and Rat Recombinant Proteins
Bárbara Frias, João Santos, Marlene Morgado, Mónica Mendes Sousa, Susannah M.Y. Gray, Karen D. McCloskey, Shelley Allen, Francisco Cruz, and Célia Duarte Cruz. The Role of Brain-Derived Neurotrophic Factor (BDNF) in the Development of Neurogenic Detrusor Overactivity (NDO). The Journal of Neuroscience, 4 February 2015, 35(5): 2146-2160; doi: 10.1523/JNEUROSCI.0373-14.2015.
...The recombinant protein TrkB-Ig2, which is an extracellular Ig-like domain of the receptor TrkB that binds to BDNF with picomolar affinity, was produced in-house and diluted in 20 mM Tris buffer, pH 8.2; 100 mM NaCl; and 10% glycerol (Banfield et al., 2001; Naylor et al., 2002). BDNF (Neuromics), saline, and TrkB-Ig2 were delivered into the subarachnoid space via osmotic mini-pumps (Alzet). Substances were delivered for 7 d (Model 2001) or 28 d (Model 2004)...
Tali Kobilo, Qing-Rong Liu, Kriti Gandhi, Mohammed Mughal, Yavin Shaham and Henriette van Praag. Running is the neurogenic and neurotrophic stimulus in environmental enrichment. doi: 10.1101/lm.2283011. Learn. Mem. 2011. 18: 605-609
... human recombinant BDNF (0.1 µg) monomer (Neuromics)...