Applications: Gene Transfer
E=ELISA; FACS; FC=Flow Cytometry; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; IP=Immunoprecipitation; NAC=Non-adherent Cell Assays; NB=Neutralization of Bioactivity; SE=Sandwich ELISA; TPE=Targeted Protein Expression; WB=Western blotting; ; AC=Adherent Cell Assays; FM=Fluorescent Micsroscopy; ; ; BSC-CM5= Biacore Sensor Chip CM5; BSM=Biosactive Small Molecule or Peptide; CDM=Cell Differentiation Media; ; ; ; ; ; Health and Fitness; ; ; DNA Extraction/Purification; ; In vivo Like Assays
SBT vectors are Sleeping Beauty transposon vectors cut down to an efficient, minimal size for ease of use. SBT Reporter Series vectors come ready for co-transfection with an SB11 transposase expression vector for easy reporter gene transfer and stable reporter gene expression in your target cells.
- Vector backbone contains two sequencing primer sites and two PvuII restriction enzyme cut sites
- Cytomegalovirus Promoter boasts high levels of gene expression in most mammalian cells, especially in neurons and embryonic stem cells.
- Enhanced Green Fluorescence Protein is a bright, rapidly active reporter gene. It is useful as both a marker for flow cytometry and fluorescence microscopy.