Applications: Gene Transfer
E=ELISA; FACS; FC=Flow Cytometry; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; IP=Immunoprecipitation; NAC=Non-adherent Cell Assays; NB=Neutralization of Bioactivity; SE=Sandwich ELISA; TPE=Targeted Protein Expression; WB=Western blotting; ; AC=Adherent Cell Assays; FM=Fluorescent Micsroscopy; ; ; BSC-CM5= Biacore Sensor Chip CM5; BSM=Biosactive Small Molecule or Peptide; CDM=Cell Differentiation Media; ; ; ; ; ; Health and Fitness; ; ; DNA Extraction/Purification; ; In vivo Like Assays
Multiple Cloning Site (MCS) ready SBT vectors are an affordable way to generate your own custom Sleeping Beauty transposon vectors. Our MCS region is compatible with several restriction enzyme sites for ease of use and is followed by a stable reporter for easy selection and use in downstream experiments. Clone and ligate your desired gene into an MCS ready SBT vector. Co-transfection with an SB11 transposase expression vector to put your gene of interest into your cells.
- Vector backbone contains two sequencing primer sites and two PvuII restriction enzyme cut sites.
- The EF-1 Alpha promoter provides a stable, high-level of expression in most all mammalian cells. A great promoter to create brightly fluorescing cells and robust drug resistant cell lines.
- The multiple cloning site (MCS) allows for the insertion of desired genes into our Sleeping Beauty transposon vectors. We follow our MCS with an IRES element to ensure consistent expression of both MCS inserted cargo and reporter genes. MCS site is prepped for digest with EcoRI and EcoRV restriction enzymes.
- Puromycin resistance gene enables drug selection of transposon containing cells.