Caroline Cvetkovic, Max H. Rich, Ritu Raman, Hyunjoon Kong & Rashid Bashir. A 3D-printed platform for modular neuromuscular motor units. Microsystems & Nanoengineering 3, Article number: 17015 (2017) doi:10.1038/micronano.2017.15

...Neuromics' GDNF used to maintain motor neurons in culture...

Joanna C. Jacob. Ethanol Reversal of Oxycodone Tolerances. Theses and Dissertations. Virginia Commonwealth University. 2017

...Glial cell line-derived neurotrophic factor (GDNF) was purchased from Neuromics (Edina, MN)....After incubation, ganglia were transferred to DMEM in a sterile 15mL conical, dissociated by triturating and centrifuged for 5 min at 1000 rpm. The supernatant was discarded and the pellet was re-suspended in neurobasal A media containing 1% fetal bovine serum, 1x B-27 supplement, 10 ng/mL GDNF, 2mM L-glutamine and 100 U/ml penicillin/streptomycin/amphotericin B (complete neuron media). Isolated cells were plated on laminin and poly-D-lysine-coated glass cover slips and maintained at 37°C in a 46 humidified 5% CO2/air incubator....

...Myenteric neurons and glia were isolated as described recently41. Briefly, after euthanizing mice, the ileum was immediately removed and placed in ice-cold Krebs solution (118 mM NaCl, 4.6 mM KCl, 1.3 mM NaH2PO4, 1.2 mM MgSO4, 25 mM NaHCO3, 11 mM glucose and 2.5 mM CaCl2) bubbled with carbogen (95% O2/5% CO2). The ileum contents were discarded by passing Krebs solution using a syringe. The ileum was then divided into short segments which were threaded longitudinally on a plastic rod through the lumen and the longitudinal muscle containing the myenteric plexus (LMMP) strips were obtained using a cotton-tipped applicator. LMMP strips were rinsed three times in 1 ml Krebs and gathered by centrifugation (350 × g, 30 sec). LMMP strips were then minced with scissors and digested in 1.3 mg/ml collagenase type II (Worthington) and 0.3 mg/ml bovine serum albumin in bubbled Krebs (37 °C) for 1 h, followed by 0.05% trypsin for 7 min. Following each digestion, cells were triturated and collected by centrifuge (350 x g for 8 min). Cells were then plated on laminin (BD Biosciences) and poly-D-lysine coated coverslips in Neurobasal A media containing B-27 supplement, 1% fetal bovine serum, 10 ng/ml glial cell line-derived neurotrophic factor (GDNF, Neuromics, Edina, MN), and penicillin/streptomycin. Half of the cell media was replaced every 2–3 days with fresh complete neuron media...

Aravind R. Gade, Minho Kang, Fayez Khan, John.R. Grider, M.Imad Damaj, William L. Dewey and Hamid I. Akbarali. Enhanced sensitivity of α3β4 nicotinic receptors in enteric neurons after chronic morphine: implication for opioid-induced constipation.The Journal of Pharmacology and Experimental Therapeutics Published online before print April 11, 2016, doi: 10.1124/jpet.116.233304

... GDNF (glial cell-derived neurotrophic factor) was purchased from Neuromics (Edina, MN)...

S. Fitting, J. Ngwainmbi, M. Kang, F. A. Khan, D. L. Stevens, W. L. Dewey, P. E. Knapp, K. F. Hauser and H. I. Akbarali. Sensitization of enteric neurons to morphine by HIV-1 Tat protein. Neurogastroenterology & Motility. DOI: 10.1111/nmo.12514.

... Cells were then plated on laminin (BD Biosciences, San Jose, CA, USA) and poly-d-lysine coated coverslips in Neurobasal A media containing B-27 supplement, 1% fetal bovine serum, 10 ng/mL glial cell line-derived neurotrophic factor (Neuromics, Edina, MN, USA)...

Tricia H. Smith, John R. Grider, William L. Dewey, Hamid I. Akbarali. Morphine Decreases Enteric Neuron Excitability via Inhibition of Sodium Channels. PLoS ONE 7(9): e45251. doi:10.1371/journal.pone.0045251.

...All chemicals and reagents were obtained from Sigma Aldrich (St Louis, MO), unless otherwise noted, except cell culture reagents, which were purchased from Gibco (Grand Island, NY). Male Swiss Webster mice (26–30 g, Harlan Sprague Dawley, Inc.) were killed by cervical dislocation. The ileum was immediately dissected and placed in ice-cold Krebs solution (in mM: 118 NaCl, 4.6 KCl, 1.3 NaH2PO4, 1.2 MgSO4 25 NaHCO3, 11 glucose and 2.5 CaCl2) bubbled with carbogen (95% O2/5% CO2). Ileal segments were threaded longitudinally on a plastic rod through the lumen and the longitudinal muscle with the myenteric plexus (LMMP) was gently removed using a cotton-tipped applicator. LMMP strips were rinsed three times in 1 ml Krebs and gathered by centrifugation (350× g, 30 s). LMMP strips were then minced with scissors and digested in 1.3 mg/ml collagenase type II (Worthington) and 0.3 mg/ml bovine serum albumin in bubbled Krebs (37°C) for 1 hour, followed by 0.05% trypsin for 7 min. Following each digestion, cells were triturated and collected by centrifuge (350× g for 8 min). Cells were then plated on laminin (BD Biosciences) and poly-D-lysine coated coverslips in Neurobasal A media containing B-27 supplement, 1% fetal bovine serum, 10 ng/ml glial cell line-derived neurotrophic factor (GDNF, Neuromics, Edina, MN), and antibiotic/antimycotic liquid. Half of the cell media was changed every 2–3 days...

Amber Young, Kristin S. Assey, Carla D. Sturkie, Franklin D. West, David W. Machacek, Steven L. Stice. Glial cell line-derived neurotrophic factor enhances in vitro differentiation of mid-/hindbrain neural progenitor cells to dopaminergic-like. doi/10.1002/jnr.22499/abstract.

...At 24 hours post-plating, the medium was changed to neural differentiation medium (proliferation medium without bFGF) plus or minus 25 ng/ml GDNF (Neuromics)...