Han Pin Pui and Yumiko Saga. Gonocytes-to-spermatogonia transition initiates prior to birth in murine testes and it requires FGF signaling. Mechanisms of Development. Volume 144, Part B, April 2017, Pages 125–139. http://dx.doi.org/10.1016/j.mod.2017.03.002

...Immunofluorescence & in situ hybridization

Whole testes or testicular explants (for culture experiments) were fixed in 4% paraformaldehyde (Nakalai)/PBS at 4 °C overnight. Then the specimens were equilibrated in sterile 30% sucrose/PBS, embedded in 22-oxacalcitriol (OCT) compound (Sakura Finetek) and sectioned to 6 μm thickness. For immunofluorescent staining, sections were pre-treated with Antigen Unmasking Solution (Vector Laboratories) or Target Retrieval Solution (Dako) at 105 °C for 5 min. This was followed by blocking with 3% skimmed milk/PBST (0.1% Tween 20/PBS) for 1 h at room temperature and incubated with primary antibodies in Can Get Signal A buffer (TOYOBO) at 4 °C overnight. The antibodies used and their dilutions were goat anti-GFRA1 (1:500, Neuromics), goat anti-RET (1:500, Neuromics), rabbit anti-PLZF (1:500, Santa Cruz Biotechnology), rabbit anti-phosphorylated Histone 3 (1:500, Cell Signaling Technology), mouse anti-phosphorylated Histone 3 (1:20, gift from Hiroshi Kimura (Hayashi-Takanaka et al., 2009)), rabbit anti-phosphorylated ERK1/2 (1:500, Cell Signaling Technology), rabbit anti-phosphorylated S6 (1:500, Cell Signaling Technology), rabbit anti-FOXO1 (1:100, Cell Signaling Technology), goat anti-E-Cadherin (1:1000, R&D Systems), rabbit anti-Laminin (1:1000, Sigma-Aldrich), rat anti-Laminin (1:100, MP Biomedicals), rabbit anti-MVH (1:500, Abcam) and rat anti-TRA98 (1:500, gift from Yoshitake Nishimune). After rinsing, the sections were incubated with appropriate secondary antibody conjugated with Alexa488 or Alexa594 or Alexa647 (1:500, Life Technologies) or CY5 (1:500, Rockland Immunochemicals) in PBST at 4 °C overnight....

Su-Yi Tsai, Rachel Sennett, Amélie Rezza, Carlos Clavel, Laura Grisanti, Roland Zemla, Sara Najam, Michael Rendl Wnt/β-catenin signaling in dermal condensates is required for hair follicle formation. Developmental Biology, Available online 3 December 2013 http://dx.doi.org/10.1016/j.ydbio.2013.11.023

...Gfra1 (goat, Neuromics)...

Aiko Sada, Kazuteru Hasegawa, Pui Han Pin, Yumiko Saga. NANOS2 Acts Downstream of Glial Cell Line-Derived Neurotrophic Factor Signaling to Suppress Differentiation of Spermatogonial Stem Cells. DOI: 10.1002/stem.790. Copyright © 2011 AlphaMed Press

...anti-GFRA1 (1:200, Neuromics, Edina, MN)...

Adrianna Kalous, Peregrine B. Osborne, Janet R. Keast. Spinal cord compression injury in adult rats initiates changes in dorsal horn remodeling that may correlate with development of neuropathic pain. The Journal of Comparative Neurology.Volume 513 Issue 6, Pages 668 - 684.Published Online: 23 Feb 2009. Copyright © 2010 Wiley-Liss, Inc., A Wiley Company .

..GFR alpha 1 (GT15004, Lot 4900084, Neuromics, Edina, MN)...

Toshihiro Uesaka, Sanjay Jain, Shigenobu Yonemura, Yasuo Uchiyama, Jeffrey Milbrandt, and Hideki Enomoto. Conditional ablation of GFR alpha1 in postmigratory enteric neurons triggers unconventional neuronal death in the colon and causes a Hirschsprung’s disease phenotype. Development 134, 2171-2181 (2007) doi:10.1242/dev.001388

...goat anti-GFR1 (1:500, Neuromics)... 

Cathy K. Naughton, Sanjay Jain, Amy M. Strickland, Akshay Gupta, and Jeffrey Milbrandt. GDNF-mediated RET signaling regulates  spermatogonial stem cell fate. DOI:10.1095/biolreprod.105.047365

. ...anti-RET, anti-GFRA1, (Neuromics, Bloomington, MN; 1:100, goat polyclonal)...