Human Cell Line Authentication
Know Your Cells
Do You Know Your Cells-Really Know Them?
Did You Know?
- Sharing cell lines between laboratories helps contribute to the estimated 18-36% of all cell lines actively in use either being cross-contaminated with other cell lines or being completely misidentified (1). Verification of cell line integrity is of utmost importance since the validity of the data obtained often depends on the identity of the cell line in use (especially when the cell lines are derived from patients with specific cancer types, disease states, etc.)
- Major journal article publishers, such as the American Association for Cancer Research (AACR) and Nature Publishing already have requirements for cell line authentication prior to publication.
- Even if you purchased your cell lines from a repository, the journal may require re-authentication prior to publishing any paper using those cell lines.
Image: Authentication Process
We are partnering with Genetica DNA Laboratories to make sure you get it right. Enjoy the advantage:
Uniquely identifies human cell lines down to the individual level (except for identical twins)
Relatively inexpensive Quick turn around time with results available in one or two days
Commercial availability of amplification kits Format is suitable to use in a universal reference database
Highly sensitive method for detecting cross-contamination between human cell lines
- Evaluates STR DNA profile consistency between low and high passage number of same cell line
(1) "The costs of using unauthenticated, over-passaged cell lines: how much more data do we need?" Hughes, P., et al. BioTechniques 43: 575-586, 2007.
|Power Plex 16 HS||16-HS-1||H||One Sample||$219|
|Power Plex 16 HS-Plus||16-HS-2||H||Two Samples||$299|