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AntibodiesKits and Reagents

Contact Information

Neuromics
5325 West 74th St., Suite 8
Edina, MN 55439

Toll free: 866-350-1500
Int'l phone: 952-374-6161
Fax: 612-677-3976

Strep-tag®

Brought to you by IBA

The Strep-tag® purification system is based on the highly selective and easily controllable interaction between the Strep-tag® II peptide and specially engineered streptavidin called Strep-Tactin®. The tagged protein binds to immobilized Strep-Tactin® during affinity purification. Physiological buffers like PBS in combination with a wide range of additives can be used. After a short washing step, gentle elution of purified recombinant protein is performed by addition of 2.5 mM desthiobiotin in the same buffer.Desthiobiotin is an inexpensive, reversibly binding and stable analog of biotin - the natural ligand of streptavidin. This competitive elution is the second step conferring specificity thus enabling unparalleled purification factors. The system is safe and easy; column regeneration and activity status are visualized by a colour change on the purification column.

Capabilities:

  • Simple one-step purification from crude lysate to 99% pure protein under physiological conditions possible.
  • High and selective binding affinity and high capacity due to special Strep-Tactin resins
  • Resins for gravity flow, low pressure or HPLC applications
  • Co-purification of non-covalently bound ligands, thus, protein-protein interaction studies are possible
  • Column regeneration and activity status is visualized by color change.

    • Brought to you by IBA

      
    Strep-tag® - Purification System - Starter Kits
    Potent Protein Purification Kits
    		Strep-tag® Related Content
    Detailed information on IBA's Strep-Tag Products
    Strep-Tactin Columns and Suspensions