Bcl-2

Recombinant Human Bcl-2 produced in E.Coli is a single, non-glycosylated polypeptide chain containing amino acids 1-218. 

Bcl is an anti-apoptic protein.It has been implicated in a number of cancers, including melanoma, breast, prostate, and lung carcinomas, as well as schizophrenia and autoimmunity. It is also thought to be involved in resistance to conventional cancer treatment. This supports a role for decreased apoptosis in the pathogenesis of cancer.

Image: BCL2 Structure and Sequence.

Applications:

• Input marker or positive control (Western Blotting).
• Function study (binding assay).

References:

1. FOWLPOX VIRUS ENCODES A BCL-2 HOMOLOGUE THAT PROTECTS CELLS FROM APOPTOTIC DEATH THROUGH INTERACTION WITH THE PRO-APOPTOTIC PROTEIN BAK.  J Virol 2007 Aug 8.
2. TRAIL activates a lysosomal pathway of apoptosis that is regulated by Bcl-2 proteins.  J Biol Chem 2007 Aug 8.
3. Bcl-2 small hairpin RNAs enhance radiation-induced apoptosis in A549 cells.  Cell Biol Int 2007 Jun 23.
4. GEA 3162, a peroxynitrite donor, induces Bcl-2-sensitive, p53-independent apoptosis in murine bone marrow cells.  Biochem Pharmacol 2007 Jun 23.
5. Bcl-2 Protein Family Members: Versatile Regulators of Calcium Signaling in Cell Survival and Apoptosis.  Annu Rev Physiol 2007 Aug 6.
6. Neuroprotective effect of nitroglycerin in a rodent model of ischemic stroke: evaluation of bcl-2 expression.  Int Rev Neurobiol 2007;82:423-35.