FACS Kits |
Our IntraCyteTM FACS products represent the next step in technology for intracellular FACS as well as neuronal and neural progenitor cell phenotyping. The kit comes complete with everything you need for intracellular FACS preparation and analysis of neural stem cells in culture.
Figure: In panel A you can see the forward scatter vs. side scatter plot from cells in culture (from our Neural Stem Cell Kit). Once gated to remove debris and necrotic cells and after setting quadrants using the IgG controls (panel B), the resulting panels show the intracellular detection of nestin and GFAP expression using IntraCyte-NSC. Note the decrease in nestin expression (both the percentage and relative fluorescence intensity in panel D and increase in GFAP expression after 5 days in NeuroCyte minimal commitment medium, compared to panel C. Also note the loss of nestin+/GFAP- population as differentiation begins, also panel C.
The complete IntraCyte FACS kit includes the following markers:
|
Antibodies |
Phenotypes |
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Nestin and GFAP |
Nestin and GFAP |
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GFAP and TUJ1/b-tubtulin III |
Neuroglial and neuronal progenitors, neurons, astrocytes |
|
CNPase and MBP |
Oligodendroglial progenitors, oligo dendrocytes |
Product Categories | |
| IntraCyte Kits | IntraCyte Reagents |