CD147

EMMPRIN (extracellular matrix metalloproteinase inducer) also called CD-147 is a 58 kDa protein with two immunoglobulin domains that was originally purified from the plasma membrane of the human LX-1 lung carcinoma cell line. EMMPRIN has been localized on the outer surface of tumor cells, where it interacts with fibroblasts to stimulate the expression of matrix metalloproteinases (MMPs). EMMPRIN expression has been observed in normal oral mucosa epithelium, kidney tissue, neoplastic oral squamous cell carcinoma, lung carcinoma, urinary bladder cancer, in situ ductal breast carcinoma and in the peripheral granulocytes of rheumatoid arthritis patients. High level of EMMPRIN expression has been shown to correlate with both MMP expression and cancer invasive potentia.

Applications:

• ELISA
• Inhibition Assays
• Western Blotting- On SDS-PAGE commassie blue stained gel, the purified recombinant protein shows a band at 84 kDa.

References:

1. [CD147 stimulates the angiogenesis in rheumatoid synovium via the activation of vascular endothelial growth factor] Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2007 May;23(5):426-8.

2. [Expression of CD147 and MMP-2 in human gliomas and its correlations with prognosis] Sichuan Da Xue Xue Bao Yi Xue Ban 2007 Jun;38(3):396-9.

3. Useful detection of CD147 (EMMPRIN) for pathological diagnosis of early hepatocellular carcinoma in needle biopsy samples. World J Gastroenterol 2007 Jun 7;13(21):2913-7.

4. HAb18G/CD147 functions in invasion and metastasis of hepatocellular carcinoma. Mol Cancer Res 2007 Jun;5(6):605-14.

5. Roles of CD147 on T lymphocytes activation and MMP-9 secretion in systemic lupus erythematosus. J Cell Mol Med 2007 Mar-Apr;11(2):339-48.

6. Monocarboxylate transporter 4 regulates maturation and trafficking of CD147 to the plasma membra ne in the metastatic breast cancer cell line MDA-MB-231. Cancer Res 2007 May 1;67(9):4182-9.

Other Reagents to Consider:

All Stem Cell Reagents

CD Antigen Proteins

All Proteins

CD and Cell Surface Marker Antibodies