96 Well Dilution Series

The Hi-Put 96 well titration plate contains serially diluted transfection reagent across the columns of the plate. Each well contains 11.7 - 0.15 μl of lipid diluted in serum-free media to a final volume of 25 μl. This format allows for the determination of the optimum amount of i-Fect reagent needed for maximal siRNA delivery. Additionally, optimal siRNA quantity may be determined by the addition of decreasing amounts of siRNA down the rows of the plate.