ELISA Kits-Neuroscience

Enzyme-linked immunosorbent assay (ELISA) kits for sensitive of neuronal and more specifically axonal injury or degeneration. Kits are detect proteins like pNF-H in the sera and spinal cord fluid (CSF) of human and animals with spinal cord and brain lesions.

Figures:  Experimental SCI results. (A) Shows average results from three rats given T11 hemisections at time 0. Blood samples were processed for ELISA at the indicated times post-injury, and error bars are standard deviations. (B) Shows data from an individual rat, not part of the data set used to generate (A), which was given a T11 hemisection and blood samples taken out to 18 days post-injury (each data point average of two determinations). (C) Shows a rat given a spinal contusion injury with serum samples taken out to 19 days. In both (B,C), 5 ll of serum was used to produce the ‘‘100,’’ plot, and the ‘‘50’’ and ‘‘25’’ plots correspond to data from serial dilutions of this material, i.e., 2.5 and 1.25 ll of serum.

Kits contains one standard ELISA 96 well plate coated with the antibody which has been blocked and so is ready to use. The plate can be separated into 8 12 well strips. Kits also includes the detection antibody and a pure standard. Also included are alkaline phosphatase conjugates, p-Nitrophenol phosphatase substrate and buffer concentrates.

We also have a variety of capture/detection antibody pairs to build your own ELISA kits please visit: Neurodegenerative Disease Research Antibodies or e-mail  pshuster@neuromics.com or call 612-801-1007 for input.