Streptamer® T-Cell Isolation/Selection
Magnetic Microbead Isolation
Streptamer® Driven T-Cell Isolation-Selection
Streptamers are used for efficient isolation of cells. T-Cells are separated from other cells by positive selection in a strong permanent magnetic field.
Figure: Example of CD8+ target cell enrichment from human PBMCs. Isolation of CD8+ cells from human PBMCs using recombinant CD8 Fab-Strep multimerized on Strep-Tactin® Magnetic Microbeads. live (PInegative) cells are shown
In a second step, the isolated cells are liberated from all labeling reagents by the addition of D-biotin (vitamin H) and washing in order to obtain a functional, completely label-free target cell preparation. The Fab-Strep ligands are engineered to have week affinity to the cognate cell surface receptor and the monomeric ligand therefore can be rapidly removed from its receptor by simple washing steps.
Figure: Receptor specific target cells are first labeled with fluorescent Fab Streptamer® (complexes of Fab-Streps and Strep-Tactin® PE or APC). In a second step, the isolated cells are liberated from all labeling reagents by addition of D-biotin (vitamin H) and washing in order to obtain a functional, completely label-free receptor specific cell preparation.
|StrepMan Magnet for 15ml and 50ml Tubes||6-5650-065||Cell Isolation-Separation||H||Cell Assays||1 Magnet||$400|
|CD3 Fab Streptamer Isolation Kit MB||6-8000-201||Cell Isolation-Separation||H||Cell Assays||1 Kit||$699|
|CD4 Fab Streptamer Isolation Kit MB||6-8000-206||Cell Isolation-Separation||H||Cell Assays||1 Kit||$699|
|CD8 Fab Streptamer Isolation Kit MB||6-8000-203||Cell Isolation-Separation||H||Cell Assays||1 Kit||$699|
|CD25 Fab Streptamer Isolation Kit MB||6-8000-207||Cell Isolation-Separation||H||Cell Assays||1 Kit||$699|
|Strep-Tactin® PE for Fab Streptamers||6-5001-010||Fluoro-Stain||H||FACS||For 1 x 10^8 cells||$295|
|Strep-Tactin® APC for Fab Streptamers||6-5011-010||Fluoro-Stain||H||FACS||For 1 x 10^8 cells||$295|