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|CH22118||100 ul||$295.00||Buy Now | Add to Cart|
Calbindin, also known as calbindin 1 or calbindin-D28k is a member of the large superfamily of cytoplasmic Ca2+ binding proteins. Calbindin-1 belongs to the subclass of these containing the "EF hand" Ca2+ binding motif originally characterized in parvalbumin. Calbindin is expressed in mammalian brain, intestine, kidney and pancreas. In the brain it is localized in certain classes of neurons, and antibodies to it are useful for identifying specific neuronal cell types. It is particularly concentrated in the dendrites and perikarya of cerebellar Purkinje cells, but is also found in many GABAergic interneurons in the cortex.
Images: Adult rat brain cortex (Left) and striatum (Right) sections (45 μM; fixed by transcardial perfusion with 4% paraformaldehyde) were stained with our Calbindin (1:1,000, red), and our monoclonal mouse Fox3/NeuN (MO22112 ). Calbindin labels a subset of sparsely-distributed interneurons (calbindin-positvie interneurons) in the cortex (Left), and more densely-distributed neurons in the striatum (Right). Since neurons also express Fox3/NeuN, calbindin-positive cells appear to be gold to yellow. Insets are high magnification images of boxed area of each image. Blue is Dapi nucleus staining.
The function of calbindin-1 appears to be primarily buffering the Ca2+ level in cells. The affinity of calbindin for Ca2+ is low at the typical resting cytoplasmic Ca2+ level of around 100 nM, and the protein only binds Ca2+ significantly when levels increase greatly. Accordingly, it is widely thought that the primary function of this protein is to act as a Ca2+ buffer. Buffering Ca2+ is important, as uncontrolled increases in the level of this cation can lead to both apoptosis due to Ca2+stimulated release of proteins from mitochondria and necrosis due to the activation of Ca2+dependent proteases.
|Primary Neurons and Astrocytes-Primary human, rat and mouse neurons and astrocytes|
Image: Western blot analysis of Calbindin. Blots of rat brain lysate (left), 0.5 µg of His-tagged recombinant proteins (right) were probed with Calbindin at 1:5,000. Lane1: Parvalbumin, Lane 2: Calretinin, Lane 3: Calbindin. In rat brain lysates, this antibody recognizes a clear band at 30 kDa which represents calbindin and it reacts only with calbindin protein, and not other calcium-binding proteins. The band at ~60 kDa is most likely the dimer of calbindin.
Below Image, courtesy of Katherine Given, University of Colorado. Protocol: Ex-vivo cerebellar slices were fixed in 4% PFA for 20min. For IHC, sections were permeabilized in 10% Triton in PBS for 20min, rinsed in PBS and then blocked in 5% NDS in PBSTx (0.3%). Primaries we apply RT overnight in 5% NDS. We used Neuromics Ckn Calbindin (CH22118) at 1:1000 (green), combined with MBP (red) and Sox10 (blue).