Catalog NumberSizePrice (USD)Shopping Cart
RA22125100 ul$295.00Buy Now | Add to Cart
 
Type: Rabbit IgG
 
Applications: ICC; IF; IHC; WB
E=ELISA; FACS; FC=Flow Cytometry; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; IP=Immunoprecipitation; NAC=Non-adherent Cell Assays; NB=Neutralization of Bioactivity; SE=Sandwich ELISA; TPE=Targeted Protein Expression; WB=Western blotting; ; AC=Adherent Cell Assays; FM=Fluorescent Micsroscopy; ; ; BSC-CM5= Biacore Sensor Chip CM5; BSM=Biosactive Small Molecule or Peptide; CDM=Cell Differentiation Media; ; ; ; ; ; Health and Fitness; ; ; DNA Extraction/Purification; ; In vivo Like Assays
Species Reactivity: H
B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins; All
Format: Whole serum - liquid
 
Immunogen: Amino acids 315-630 of the human protein
 
Description/Data:
IHC Image of Nestin - RA22125

The protein proved to be an unusual member of the intermediate filament family, containing an α-helical region homologous to that found in keratin and neurofilament subunits. The Human Genome Organization (HUGO) Gene Nomenclature Committee (HGNC) name for Nestin is NES.

Image: SH-SY5Y neuroblastoma cells were stained with Nestin (RA221025) at 1:5,000 (red) and CH22108 against vimentin (green) at 1: 10,000. Vimentin is main partner of nestin forming heterdimers and further polymerizing to form intermediate filaments. Co-localization of vimentin and nestin in cells was shown in yellow. Blue is DAPI staining of DNA.

Nestin is relatively poorly conserved in protein sequence across species boundaries, so that the mouse and human proteins have an overall identity of only 62%. As a result antibodies to the human protein often fail to recognize the rodent homologue and vice versa. Antibodies to nestin are widely used to identify neural stem cells. This antibody was raised in rabbit against recombinant human nestin from aa 315-630, which has only 55% identity to homologous mouse sequence. Nestin (RA22125) therefore recognizes nestin protein in human, but not in rodent.