Strep/6xHistidine Starter Kit H-PR

For purification of double tag proteins
Strep/6XHistidine Purification Protocol(pdf - 1020Kb)
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2-1117-0002X1 ml Columns$395.00Buy Now | Add to Cart
 
Type: Kit
 
Applications: Protein Purification
ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; WB=Western blotting; FC=Flow Cytometry; IP=Immunoprecipitation; E=ELISA; NB=Neutralization of Bioactivity; FACS; FM=Fluorescent Micsroscopy; ; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; TPE=Targeted Protein Expression; ; ; AC=Adherent Cell Assays; ; ; NAC=Non-adherent Cell Assays; ; ; BSC-CM5= Biacore Sensor Chip CM5; ;
Species Reactivity: Not Applicable
B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins
Description/Data:
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This unique new Starter Kit contains all reagents essential for the native purification of a double tag protein containing Strep-tag and 6xHistidine-tag. The first purification is performed on a Ni-NTA cartridge H-PR while the second purification uses a Strep-Tactin® Superflow® high capacity cartridge H-PR.

Kit Contents:
  • 1 Strep-Tactin® Superflow® high capacity cartridge H-PR (1 ml)
  • 1 Ni-NTA Superflow cartridge H-PR (1 ml)
  • 1 coupling adapter Strep-Tactin Washing Buffer ("Buffer W")
  • Strep-Tactin Elution Buffer ("Buffer E")
  • Strep-Tactin Regeneration Buffer ("Buffer R")
  • Ni-NTA Equilibration Buffer ("Lysis Buffer")
  • Ni-NTA Washing Buffer ("Wash Buffer")
  • Ni-NTA Elution Buffer ("Elution Buffer")
  • NiSO4 * 6H2O for recharging the Ni-NTA resin
  • Anhydrotetracycline for induction of expression Control plasmid with 16,6 kDa protein insert Strep-Tactin
  • HRP conjugate for Western blot detection
  • Comprehensive Manual