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MO22114100 ul$225.00   $275.00Buy Now | Add to Cart
 
Type: Mouse IgG
 
Applications: ICC; IF; WB
E=ELISA; FACS; FC=Flow Cytometry; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; IP=Immunoprecipitation; NAC=Non-adherent Cell Assays; NB=Neutralization of Bioactivity; SE=Sandwich ELISA; TPE=Targeted Protein Expression; WB=Western blotting; ; AC=Adherent Cell Assays; FM=Fluorescent Micsroscopy; ; ; BSC-CM5= Biacore Sensor Chip CM5; BSM=Biosactive Small Molecule or Peptide; CDM=Cell Differentiation Media; ; ; ; ; ; Health and Fitness; ; ; DNA Extraction/Purification; ; In vivo Like Assays
Species Reactivity: H
B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins; All
Format: Supernatant - liquid
 
Description/Data:
Picture

TAF15 is a member of a family of 3 closely related mammalian RNA binding proteins, the other members being FUS/TLS and EWSR1. TDP43 is a more distant relative of these three. The protein is also known as RBP56, for RNA binding protein 56kDa. As with the other members of this family, TAF15 contains one copy of the ~90 amino acid RRM domain. RRM is an acronym for RNA Recognition Motif, and this domain is found in many proteins which bind single stranded RNA and DNA. Like TDP43, Fus/TLS and EWS, TAF15 is widely expressed in tissues and is localized primarily in the nucleus of cells. It was recently shown that point mutations in TAF15 may be associated with some familial forms of Amyotrophic Lateral Sclerosis (ALS).

Image: shows HeLa cell cultures stained with TAF15 antibody (green) and chicken antibody to Vimentin (red). TAF15  reveals a granular nuclear localization typical of many RNA associated proteins. Blue is a DNA stain.

Picture[2]

Image: Blots of crude HeLa cell homogenate blotted with TAF15. The monoclonal antibody binds strongly and cleanly to a band at about 68 kDa.