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GT41028100 ug$250.00   $325.00Buy Now | Add to Cart
P41028100 ug Blocking Peptide$145.00Buy Now | Add to Cart
 
Type: Goat IgG
 
Applications: IHC; WB; IP
E=ELISA; FACS; FC=Flow Cytometry; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; IP=Immunoprecipitation; NAC=Non-adherent Cell Assays; NB=Neutralization of Bioactivity; SE=Sandwich ELISA; TPE=Targeted Protein Expression; WB=Western blotting; ; AC=Adherent Cell Assays; FM=Fluorescent Micsroscopy; ; ; BSC-CM5= Biacore Sensor Chip CM5; BSM=Biosactive Small Molecule or Peptide; CDM=Cell Differentiation Media; ; ; ; ; ; Health and Fitness; ; ; DNA Extraction/Purification; ; In vivo Like Assays
Species Reactivity: H; M; R
B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins; All
Format: Affinity Purified - liquid
 
Immunogen: Peptide with sequence DSGNHCFKVYRYLQ, from the C Terminus of the TRIM2 protein sequence according to NP_056086.2; NP_001123539.1
 
Description/Data:
Picture

TRIM RING finger protein TRIM2, a ubiquitin ligase, is highly expressed in the nervous system. It binds to neurofilament light subunit (NF-L) and regulates NF-L ubiquitination. Deficiencies in TRIM2 increases NF-L level in axons and NF-L-filled axonal swellings in cerebellum, retina, spinal cord, and cerebral cortex.

Image: TRIM2 (dilution: 6.3 µg/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Note cytoplasm staining in neuronal cell bodies. Protocol on datasheet.

These deficiencies eventually lead to neurodegeneration, tremors and ataxia. It is proposed that TRIM2 has neuroprotection properties.

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Image: TRIM2 (dilution: 0.5 µg/ml) staining of Rat Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.