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GT15245100 ug$365.00Buy Now | Add to Cart
 
Type: Goat IgG
 
Applications: ICC; WB; IP
E=ELISA; FACS; FC=Flow Cytometry; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; IP=Immunoprecipitation; NAC=Non-adherent Cell Assays; NB=Neutralization of Bioactivity; SE=Sandwich ELISA; TPE=Targeted Protein Expression; WB=Western blotting; ; AC=Adherent Cell Assays; FM=Fluorescent Micsroscopy; ; ; BSC-CM5= Biacore Sensor Chip CM5; BSM=Biosactive Small Molecule or Peptide; CDM=Cell Differentiation Media; ; ; ; ; ; Health and Fitness; ; ; DNA Extraction/Purification; ; In vivo Like Assays
Species Reactivity: H
B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins; All
Format: Affinity Purified - liquid
 
Immunogen: E. coli-derived recombinant human Snail. Pro2-Arg264 Accession Number O95863
 
Description/Data:
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Snail or Snail-1 is a zinc-finger transcription factor that is associated with neural crest formation in chick, and left-right asymmetry development in mice. Snail is particularly known to repress E-Cadherin expression, regulating epithelial-to-mesenchymal transitions and cell migration. Snail family members influence cell behaviour during development and diseases such as metastatic cancer, where they act primarily as survival factors and inducers of cell movement.

Image:Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin for 30 minutes was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Snail/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human Snail Antigen Affinity-purified Polyclonal Antibody or control antibody for 15 minutes in a n ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody. Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid and DNA was purified using chelating resin solution. The E-Cadherin promoter was detected by standard PCR.

 

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Image: Western blot shows lysates of A549 human lung carcinoma cell line and JEG‑3 human epithelial choriocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Snail Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for Snail at approximately 29 kDa (as indicated).