Picture

The main function of fibroblasts is to maintain the structural integrity of connective tissues by continuously secreting precursors of the extracellular matrix. Like other cells of connective tissue, fibroblasts are derived from primitive mesenchyme. Thus they express the intermediate filament protein vimentin, a feature used as a marker to distinguish their mesodermal origin.

Figure: Continuous expansion of human fibroblasts. Cells expanded through 16 passages. Fibroblast were plated at 5,000 cells/cm2  in Greiner Bio-one T25 flasks maintained in VitroPlus III, low serum media in a reduced oxygen environment (5% O2, 5% CO2, 90% N2) at 37OF in a humidified chamber. The pancreatic fibroblasts were detached using by incubation in AccutaseTM they were approximately at 80-90% confluency and counted using a Beckman-Coulter Z2.

In addition forming and maintaining extracellular matrices, they also regulate interstitial fluid volume and pressure, and wound healing. Many diseases are associated with dysregulation of the injury repair response and fibroblast function, leading to increased or decreased deposition of extracellular matrix proteins, altered tissue architecture, impaired function and in some cases significant morbidity and mortality. This includes association with cancer cells at all stages of cancer progression (cancer associated fibroblasts).

Culturing, Sub-Culturing and Cryopreservation Protocol

 

NameCatalog #TypeSpeciesApplicationsSizePrice
Native Human FibroblastsSC00A5Primary CellsHCell Assays500,000 Cells$495
Human Fibroblasts-GFP ExpressingGFP07Primary CellsHCell Assays500,000 Cells in T25 flask$999
VitroPlus III, low serum, completePC00B1-500Cell Growth Media500 ml$184