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ELH-Eotaxin-0011 Plate Kit$379.00Buy Now | Add to Cart
ELH-Eotaxin-0022 Plate Kit$679.00Buy Now | Add to Cart
ELH-Eotaxin-0025 Plate Kit$1,499.00Buy Now | Add to Cart
 
Type: Kit
 
Applications: E
E=ELISA; FACS; FC=Flow Cytometry; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; IP=Immunoprecipitation; NAC=Non-adherent Cell Assays; NB=Neutralization of Bioactivity; SE=Sandwich ELISA; TPE=Targeted Protein Expression; WB=Western blotting; ; AC=Adherent Cell Assays; FM=Fluorescent Micsroscopy; ; ; BSC-CM5= Biacore Sensor Chip CM5; BSM=Biosactive Small Molecule or Peptide; CDM=Cell Differentiation Media; ; ; ; ; ; Health and Fitness; ; ; DNA Extraction/Purification; ; In vivo Like Assays
Species Reactivity: H
B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins; All
Description/Data:
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Sensitive, Specific and Cost Effective

Eotaxin-1 or CCL11 is a small cytokine belonging to the CC chemokine family. CCL11 selectively recruits eosinophils by inducing their chemotaxis, and therefore, is implicated in immune and allergic responses. Recently, it has shown to protect against neuroinflammation in rats.

It has recently identified as a new diagnostic serum marker for prostate cancer and could help distinguish between prostatic enlargement and prostate cancer among men with normal range serum PSA values. Eotaxin-1/CCL11 could play a role in decreasing neurogenesis and cognitive performance

Eotaxin-1 Standard Curve

Suggested Application: Quantitative Protein Detection; Establishing Normal Range; Validation of Antibody Array Results.

Kit Components:
·   Pre-Coated 96-Well Strip Microplate
·   Wash Buffer
·   Stop Solution
·   Assay Diluent(s)
·   Lyophilized Standard
·   Biotinylated Detection Antibody
·   Streptavidin-Conjugated HRP
·   TMB One-Step Substrate
Other Materials Required:
·     Distilled or deionized water
·     Precision pipettes to deliver 2 μl to 1 ml volumes
·     Adjustable 1-25 ml pipettes for reagent preparation
·     100 ml and 1 liter graduated cylinders
·     Tubes to prepare standard and sample dilutions
·     Absorbent paper
·     Microplate reader capable of measuring absorbance at 450nm
·     Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline:
1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C.
3. Add 100 μl of prepared biotin antibody to each well. Incubate 1 h at RT.
4. Add 100 μl of prepared Streptavidin solution to each well. Incubate 45 min at RT.
5. Add 100 μl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT.
6. Add 50 μl of Stop Solution to each well. Read at 450 nm immediately.