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RA22119100 ul$295.00Buy Now | Add to Cart
 
Type: Rabbit IgG
 
Applications: IF; WB
E=ELISA; FACS; FC=Flow Cytometry; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; IP=Immunoprecipitation; NAC=Non-adherent Cell Assays; NB=Neutralization of Bioactivity; SE=Sandwich ELISA; TPE=Targeted Protein Expression; WB=Western blotting; ; AC=Adherent Cell Assays; FM=Fluorescent Micsroscopy; ; ; BSC-CM5= Biacore Sensor Chip CM5; BSM=Biosactive Small Molecule or Peptide; CDM=Cell Differentiation Media; ; ; ; ; ; Health and Fitness; ; ; DNA Extraction/Purification; ; In vivo Like Assays
Species Reactivity: H; M; Pr; R
B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins; All
Format: Whole serum - liquid
 
Immunogen: Full length recombinant human ALDH1L1 expressed and purified from E. coli.
 
Description/Data:
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ALDH1L1 catalyzes the conversion of 10-formyltetrahydrofolate, nicotinamide adenine dinucleotide phosphate (NADP+), and water to tetrahydrofolate, NADPH, and carbon dioxide. ALDH1L1 expression is tissue-specific and is highly expressed in the liver.

Image: Neuron-glial cell mixture cultures stained with ALDH1L1 (red) and our monoclonal antibody against GFAP (green). Blue is a DNA stain. ALDH1L1 stains astrocytes and excludes from neuron cells. ALDH1L1 stains the astrocytes cell body and processes, whereas GFAP labels the intermediate filament of the cytoskeleton in subset of astrocytes. Astrocytes are positive for both ALDH1L1 and GFAP appear yellow. ALDH1L1 also labels many astrocytes not labeled by GFAP, which appear as red.

In an earlier study, Cahoy et al. applied FACS (Fluorescent-Activated Cell Sorting) to isolate astrocytes from EGFP (Enhanced Green Fluorescent Protein) transgenic-mouse, then created a transcriptome database of the expression levels of 20,000 genes by gene profiling of neurons, astrocytes and oligodedrocytes using Affymetrix GeneChip Arrays. They identified ALDH1L1 as a highly and specifically expressed gene in astrocytes. ALDH1L1 is more widely expressed throughout the brains, while astrocyte marker GFAP shows more predominant expression in white matter. In addition, loss of function or expression of  ALDH1L1 is associated with decreased apoptosis, increased cell motility, and cancer progression, suggesting its role as a biomarker and a target in cancer therapy

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Image: Image: Blot of rat liver tissure homogenates blotted with ALDH1L1. The antibody binds strongly a band at ~100 kDa.