Catalog NumberSizePrice (USD)Shopping Cart
HEC02500,000+ Cells frozen$789.00Buy Now | Add to Cart
HEC02-T25500,000+ Cells in T25 Flask$789.00Buy Now | Add to Cart
 
Type: Primary Cells
 
Applications: Cell Assays
E=ELISA; FACS; FC=Flow Cytometry; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; IP=Immunoprecipitation; NAC=Non-adherent Cell Assays; NB=Neutralization of Bioactivity; SE=Sandwich ELISA; TPE=Targeted Protein Expression; WB=Western blotting; ; AC=Adherent Cell Assays; FM=Fluorescent Micsroscopy; ; ; BSC-CM5= Biacore Sensor Chip CM5; BSM=Biosactive Small Molecule or Peptide; CDM=Cell Differentiation Media; ; ; ; ; ; Health and Fitness; ; ; DNA Extraction/Purification; ; In vivo Like Assays
Species Reactivity: H
B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins; All
Description/Data:
Human Brain Microvascular Endothelial Cell or HBMECs Image

Culturing Protocol - T25 Flask 

HBMECs were isolated from normal human brain tissue. Passage 2, cells are ship on dry ice in a vial or in proliferating culture with a confluency of >90 %*.  ENDO-Growth medium containing 5% serum and growth supplements are recommended for culture. Cells have an average additional population doubling levels >15 when cultured.

Image: Human Brain Microvascular Endothelial Cells in Culture.

Characterization of the cells
  • Cytoplasmic VWF / Factor VIII: >95% positive by immunofluorescence
  • Cytoplasmic uptake of Di-I-Ac-LDL: >95% positive by immunofluorescence
  • Cytoplasmic PECAM1 >95% positive by immunofluorescence 

HBMECs are negative for HIV-1, HBV, HCV, and mycoplasma.

Date of Expiration: Guaranteed for up to 6 months from date of shipping when stored in liquid nitrogen.

**cells are shipped at 90% confluence. However, confluence can be lost during shipping. Please allow for 1-4 days to reach 90% confluence again. 

Recommended Products

ENDO-Growth Media – MED001Contains 475 ml of ENDO-Basal Media and 25 ml of ENDO-Growth Supplement combined. Which is freshly prepared for your convenience                                                                                                                OR                                                                                                                                                                                                                ENDO-Growth Kit – EGK001: Contains 475 of ENDO-Basal Media and 25 ml of ENDO-Growth Supplement separately to be mixed to make growth media

 Smooth Coat Solution – SC300 Biocompatible complex of extracellular matrix binding solution
OR
AlphaBioCoat Solution – AC001: Premium Smooth Coat Solution. Biocompatible complex of extracellular matrix binding solution with growth factors. Ideal for culturing cells from frozen.

Cell Detachment Solution – ADF001: Contains protease and collagenase activities in an isotonic, phosphate buffer solution with EDTA to detach primary cells and cell lines

1X Phosphate Buffer Solution - PBS300