|Catalog Number||Size||Price (USD)||Shopping Cart|
|ELH-Insulin-001||1 Plate Kit||$379.00||Buy Now | Add to Cart|
|ELH-Insulin-002||2 Plate Kit||$679.00||Buy Now | Add to Cart|
|ELH-Insulin-003||5 Plate Kit||$1,499.00||Buy Now | Add to Cart|
Sensitive, Specific and Cost Effective
Human Insulin ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Insulin and Proinsulin in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human Insulin coated on a 96-well plate. Standards and samples are pipetted into the wells and Insulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Insulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Insulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Figure: Correlations between serum and seminal insulin (A) and leptin (B) concentrations within the cohort. A significant correlation (r2 = 0.83) between serum and seminal insulin concentrations (A) was found; similarly, a significant correlation (r2 = 0.52) between serum and seminal leptin (B) concentrations was found. The figures show the distribution of the non-obese and obese groups, reflecting a clear increase in serum and seminal concentrations of both hormones with obesity. x = participants in the non-obese group (circled with the dash lines); □ = participants in the obese group (circled with the dotted line). Leisegang et al. Reproductive Biology and Endocrinology 2014 12:34 doi:10.1186/1477-7827-12-34.
Suggested Application: Quantitative Protein Detection; Establishing Normal Range; Validation of Antibody Array Results.