Catalog NumberSizePrice (USD)Shopping Cart
ELH-Insulin-0011 Plate Kit$379.00Buy Now | Add to Cart
ELH-Insulin-0022 Plate Kit$679.00Buy Now | Add to Cart
ELH-Insulin-0035 Plate Kit$1,499.00Buy Now | Add to Cart
 
Type: Kit
 
Applications: E
E=ELISA; FACS; FC=Flow Cytometry; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; IP=Immunoprecipitation; NAC=Non-adherent Cell Assays; NB=Neutralization of Bioactivity; SE=Sandwich ELISA; TPE=Targeted Protein Expression; WB=Western blotting; ; AC=Adherent Cell Assays; FM=Fluorescent Micsroscopy; ; ; BSC-CM5= Biacore Sensor Chip CM5; BSM=Biosactive Small Molecule or Peptide; CDM=Cell Differentiation Media; ; ; ; ; ; Health and Fitness; ; ; DNA Extraction/Purification; ; In vivo Like Assays
Species Reactivity: H
B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins; All
Description/Data:

Sensitive, Specific and Cost Effective

Human Insulin ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Insulin and Proinsulin in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human Insulin coated on a 96-well plate. Standards and samples are pipetted into the wells and Insulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Insulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Insulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Insulin ELISA Kit Publication

Figure: Correlations between serum and seminal insulin (A) and leptin (B) concentrations within the cohort. A significant correlation (r2 = 0.83) between serum and seminal insulin concentrations (A) was found; similarly, a significant correlation (r2 = 0.52) between serum and seminal leptin (B) concentrations was found. The figures show the distribution of the non-obese and obese groups, reflecting a clear increase in serum and seminal concentrations of both hormones with obesity. x = participants in the non-obese group (circled with the dash lines); □ = participants in the obese group (circled with the dotted line). Leisegang et al. Reproductive Biology and Endocrinology 2014 12:34 doi:10.1186/1477-7827-12-34.

Suggested Application: Quantitative Protein Detection; Establishing Normal Range; Validation of Antibody Array Results.

Kit Components:
·   Pre-Coated 96-Well Strip Microplate
·   Wash Buffer
·   Stop Solution
·   Assay Diluent(s)
·   Lyophilized Standard
·   Biotinylated Detection Antibody
·   Streptavidin-Conjugated HRP
·   TMB One-Step Substrate
Other Materials Required:
·     Distilled or deionized water
·     Precision pipettes to deliver 2 μl to 1 ml volumes
·     Adjustable 1-25 ml pipettes for reagent preparation
·     100 ml and 1 liter graduated cylinders
·     Tubes to prepare standard and sample dilutions
·     Absorbent paper
·     Microplate reader capable of measuring absorbance at 450nm
·     Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline:
1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C.
3. Add 100 μl of prepared biotin antibody to each well. Incubate 1 h at RT.
4. Add 100 μl of prepared Streptavidin solution to each well. Incubate 45 min at RT.
5. Add 100 μl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT.
6. Add 50 μl of Stop Solution to each well. Read at 450 nm immediately.