|Catalog Number||Size||Price (USD)||Shopping Cart|
|ELH-IL12p40-001||1 Plate Kit||$379.00||Buy Now | Add to Cart|
|ELH-IL12p40-002||2 Plate Kit||$679.00||Buy Now | Add to Cart|
|ELH-IL12p40-003||5 Plate Kit||$1,449.00||Buy Now | Add to Cart|
Sensitive, Specific and Cost Effective
Human IL-12p40 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IL-12p40 and ProIL-12p40 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human IL-12p40 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-12p40 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-12p40 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-12p40 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Suggested Application: Quantitative Protein Detection; Establishing Normal Range; Validation of Antibody Array Results.