GluR4/AMPA4

Affinity Purified Goat Antibody
DATASHEET(pdf - 113Kb)
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Type: Goat IgG
 
Applications: IHC; WB; E
E=ELISA; FC=Flow Cytometry; ICC=Immunocytochemistry; IF=Immunofluorescence; IP=Immunoprecipitation; IHC=Immunohistochemistry; SE=Sandwich ELISA; WB=Western blotting; NB=Neutralization of Bioactivity; FACS; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; BSM=Biosactive Small Molecule or Peptide; HPLC=High Performance Liquid Chromatography; TPE=Targeted Protein Expression; AC=Adherent Cell Assays; NAC=Non-adherent Cell Assays; CDM=Cell Differentiation Media; BSC-CM5= Biacore Sensor Chip CM5; FM=Fluorescent Micsroscopy; ; ; ; ; ; ; ; ; ;
Species Reactivity: B; H; M; R
B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins; All
Format: Affinity Purified - liquid
 
Immunogen: Synthetic peptide: C-KKLDQREYPGSETP, from the internal region of the protein sequence according to NP_000820.3; NP_001070711.1; NP_001070712.1.
 
Description/Data:
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Human glutamate receptor 4 (GRIA4) is a new member of the family of ionotropic glutamate receptors which are the predominant excitatory neurotransmitter receptors in the mammalian brain. Binding studies showed that human GluR4 transfected into simian kidney cells (COS-1) exhibits high specific binding for[3H](RS)-alpha-amino- 3-hydroxy-5-methylisoxazole-4-propionic acid ([3H]AMPA) but not [3H]kainate. Ion substitution experiments indicate that hGluR4 receptor-linked ion channels in their homomeric state are permeable to both CA2+ and Na+ ions. Immunoprecipitation studies suggest that GRIA1 exists in situ in the form of a pentamer.

Image: GluR4 staining of human brain tissue, cortex (formalin-fixed paraffin-embedded) at 5 ug/ml followed by biotinylated horse anti-goat IgG secondary antibody,  alkaline phosphatase-streptavidin and chromogen. Protocol on data-sheet.

Nociceptors, involved primarily in the mediation of neuropathic pain, may be presynaptically modulated by GluR4-containing AMPA receptors.

More Links:

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Metabotropic Glutamate Receptor Antibodies (mGluRs)

Neurotransmission Research Antibodies                             

Pain and Inflammation Research Antibodies

Primary Neurons and Astrocytes-Primary human, rat and mouse neurons and astrocytes
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Image: GluR4 (0.3µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.