Neuromics' 2012-2013 Newsletters

  Neuromics

May 2013 News

Tested, Characterized and Research Ready

Better Cell Cultures=Lower Costs
A common Neuromics' theme is harnessing the power of cellsTM. The raw cost of the cells are often the biggest consideration. We encourage our customers to focus on true costs. These include the #of cells (how many times can they be passaged?), culture viability (how long do the cells live) and bioactivity (how closely do cultures mimic in vivo behavior?). I would like to present a publication and presentation confirms our competitive advantage when analyzing true costs.

 

Dr. Randen Patterson and his team at UC Davis have developed new culturing techniques using our e18 Rat Primary Hippocampal Neurons. They have developed a protocol that allows for culturing of E18 hippocampal neurons at high densities for more than 120 days. These cultured hippocampal neurons are (i) well differentiated with high numbers of synapses, (ii) anchored securely to their substrate, (iii) have high levels of functional connectivity, and (iv) form dense multi-layered cellular networks: 10.1371/journal.pone.0058996
Publications and Presentations Highlights

Todd GK, Boosalis CA, Burzycki AA, Steinman MQ, Hester LD, et al. (2013) Towards Neuronal Organoids: A Method for Long-Term Culturing of High-Density Hippocampal Neurons.PLoS ONE 8(4): e58996. doi:10.1371/journal.pone.0058996

Images: 30 DIV Hippocampal Cultures. Immunofluorescence detection of MAP-2 (green) and GFAP (red) in 30 DIV (A–C) cultured E18 hippocampal cells using a 40X objective. These images (A and B) clearly depict the intimate physical contact between glia processes and dendritic arbors. Under closer examination (CI and CII), it is clear that the dendrites have grown both below (blue arrows) and above (white arrows) glial processes, forming a highly interconnected three-dimensional network by 30 DIV. doi:10.1371/journal.pone.0058996.g004.

 Neurons-Glia 
  • Cells are differentiated from human MSCs-Competitive to IPSCs since there is no reprogramming nor epigenetic issues intrinsic to IPSCs
  • Cost-effective due to manufacturing scalability Direct purchase without licensing requirement Highly experienced team with extensive technical and biopharma partnership experience.
  • Cell therapy application as well as discovery.

 

   HTS
Testimonial: "We tested the effects of MSCGroTM defined medium using several different lots of human adult primary stem cells and found that MSCGro supports a more robust proliferation rate than normal undefined media. This provided shorter doubling times and increased cellular yield, and maintained the cells in an undifferentiated state. We also found that MSCGro medium is stable under normal laboratory conditions for an extended time period compared to other defined media." Ben Buehrer, VP and CSO, Zen-Bio
Promos and New Products Highlights
 Save on Cells Featured New Products

Image: Zebularine inhibits tumor cell proliferation & Induces Differentiation of MSCs into Cardiomyocytes

  Zebularine

Here's to new and exciting discoveries.

Pete Shuster-CEO and Owner/direct phone: 612-801-1007
pshuster@neuromics.com

 

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April 2013 NewsSTEMEZ(TM) hNP1 Human Neural Progenitors and hN2 Primary Neurons Differentiation and Expression
G-protein Coupled Receptor Expression Patterns. This information was presented by Dr. Steve Stice and his team and the 2008 Neuroscience Conference.
RNAi Gene Silencing