Type: Mouse IgG
Applications: IHC; WB
ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; WB=Western blotting; FC=Flow Cytometry; IP=Immunoprecipitation; E=ELISA; NB=Neutralization of Bioactivity; FACS; FM=Fluorescent Micsroscopy; ; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; TPE=Targeted Protein Expression; ; ; AC=Adherent Cell Assays; ; ; NAC=Non-adherent Cell Assays; ; ; BSC-CM5= Biacore Sensor Chip CM5; ;
Species Reactivity: H; M; R
B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins
Format: Supernatant - liquid
Immunogen: Prokaryotic recombinant protein of 31kD corresponding to the C-terminus of the heat shock protein 105 molecule. Prokaryotic recombinant protein of 31kD corresponding to the C-terminus of the heat shock protein 105 molecule.Clone: 58F12
Description/Data:
Heat shock protein 105 (Hsp105) exists as two isoforms; alpha and beta which belong to the Hsp105/Hsp110 protein family. Hsp105 acts as both a chaperone to prevent thermal aggregation of proteins and as a regulator of mammalian cells. The Hsp105 isoforms are reported to be found in the cytoplasm but not in the nucleoli under non-stressed and stressed condit-ions. In rodents, Hsp105 isoforms are reported to be moderately expressed in the adrenal glands, spleen, liver and heart and both are markedly increased after heat shock. In the testis, Hsp105 is specifically localized in the cytoplasm of germ cells but may translocate to the nucleus after heat shock. The most abundant expression of Hsp105 occurs in the brain with nuclear and cytoplasmic expression in nearly all neurons, oligodendrocytes, microglia and astrocytes. Increased expression reported during embryogenesis suggests that Hsp105 may have an important role during mouse development.
Image: Heat shock protein 105 staining of Human Cerebellum Tissue. Paraffin section. Note intense cytoplasmic and nuclear staining of Purkinje cells and neuronal processes.
