MATra-s-Immobilizer

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Description/Data:
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Suspension cells have to be made adherent first by incubating them with the reagent MATra-S Immobilizer which contains magnetic nanoparticles (MagTag™).

Image: Universal magnet plate (8 x 13 cm) with collection of different culture plates and MATra reagents.

Comprehensive Protocol for All MATra-Three different procedures according to different initial situations can be followed to apply MATra:
  • MATra for adherent cells Magnet Assisted Transfection for adherent cells. The nucleic acid has to be combined with MATra-A (plasmid DNA) or MATra-si (siRNA) Reagent and MATra can be performed directly .
  • MATra for suspension cells-Magnet Assisted Transfection for suspension cells. Suspension cells have to be made adherent first by incubating them with the magnetic reagent MATra-S Immobilizer or by using polylysine plates. Then MATra-A or MATra-si Reagent loaded with the nucleic acid can be applied and MATra can be performed.

  • MATra Lipofection for adherent and suspension cells-Transfection with common lipid based (e.g. i-Fect ™, pn-Fect™ , IBAfect , Lipofectamine or Fugene) or polycationic reagents (e.g. ExGen500 or Superfect) can be enhanced by magnetic assistance. In this case, the nucleic acid is combined with MA Lipofection Enhancer in the presence of the common lipofection reagent. In addition, the MA Lipofection Enhancer can be used for viral transfection.

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