hN2™ Human Neurons Discovery & HT Screening Kits

Customer Testimonial: "The cells grew very well, no problems at all. I will try to get some more images together over the next few days". Dr. Gerry Shaw, University of Florida. 

hN2™ Kits can generate many neuronal phenotypes, including cells positive for the following: HB9 for motoneurons, ChAT for cholinergic neurons, DAT for dopaminergic neurons, GABA for GABAergic neurons, (Images), all cells relevant to studying various diseases as well as usable in assays for general toxicity not relevant to cellular phenotype. For The hN2™ Discover Kits, the cells have undergone two weeks of differentiation. The universal neural cell marker Tuj (beta tubulin III) indicates that >80% of the hN2™cells are neural. The hN2™cells possess electrically active membranes and the hN2™cells produce uniform responses in FLIPR glutamatergic pharmacology studies.

Images: Neural phenotypes derived from hN2 cell lines. (A) Phase contrast image of differentiated culture. (B) Network including post-mitotic motoneurons (HB9). (C) Cholinergic neuron. (D) Tuj-1 positive cells that are DAT-positive (dopamine transporter; closed arrow) and DAT-negative (open arrow). (E) Gabaergic neurons, inset illustrates GABA in axon, but not the dendrites (arrow). hN2 Cells-Electro Phys Data. 

Note: hN2™ HTS Kit is an excellent choice for high-throughput (96- and 384-well) formatted assays.