Strep-tag® - Purification System - Starter Kits

Potent Protein Purification Kits

Strep-tag Starter Kit
1 purification column

Strep-tag Starter Kit 3C
3 different purification columns

Strep-tag Starter Kit "Cartridge H-PR" Superflow

Strep-tag Starter Kit "Cartridge" Macroprep

Kit contents:

Kit contents:

Kit contents:

Kit contents:

1 ready-to-use column with Strep-Tactin Sepharose, 1 ml

For Strep and 6X Histidine Tagged Proteins use: Strep/6xHistidine Starter Kit H-PR

3 ready-to-use columns with Strep-Tactin Sepharose, Strep-Tactin MacroPrep and Strep-Tactin Superflow, respectively (1ml each)

1 Strep-Tactin Superflow H-PR cartridge (1 ml); with 4 adapter sets for connection to Luer-lock syringes and various chromatography systems and workstations

 1 Strep-Tactin MacroPrep cartridge, 1 ml; with 4 adapter sets for connection to peristaltic pump chromatography systems or FPLC/HPLC workstations

Included in  Strep-Tag kits (sufficient for 8 applications):
  • control plasmid with 15 kD protein insert anhydrotetracycline for induction of expression
  • fractionation buffer for the preparation of a periplasmic extract
  • washing buffer for column chromatography or for the preparation of a cytoplasmic extract
    		•
    • elution buffer for displacing the Strep-tag protein from the column column regeneration buffer (with HABA)
    • Strep-Tactin horse radish peroxidase (HRP) conjugate for Western blot detection
    • Comprehensive manual
    Included in Strep/6xHistidine Starter Kit H-PR  
  • 1 Strep-Tactin® Superflow® high capacity cartridge H-PR (1 ml)
  • 1 Ni-NTA Superflow cartridge H-PR (1 ml)                                               
  • 1 coupling adapter Strep-Tactin Washing Buffer ("Buffer W")
  • Strep-Tactin Elution Buffer ("Buffer E")
  • Strep-Tactin Regeneration Buffer ("Buffer R")
  • Ni-NTA Equilibration Buffer ("Lysis Buffer")
    		•
  • Ni-NTA Washing Buffer ("Wash Buffer")
  • Ni-NTA Elution Buffer ("Elution Buffer")
  • NiSO4 * 6H2O for recharging the Ni-NTA resin
  • Anhydrotetracycline for induction of expression Control plasmid with 16,6 kDa protein insert Strep-Tactin
  • HRP conjugate for Western blot detection
  • Comprehensive Manual
    		•
    NameCatalog #TypeSpeciesApplicationsSizePrice
    Strep-tag Starter Kit2-1101-000KitNot ApplicableProtein Purification1 Column$250
    Strep-tag Starter Kit 3C2-1102-001KitNot ApplicableProtein Purification3 Columns$475
    Strep-tag Starter Kit Cartridge H-PR Superflow2-1115-000KitNot ApplicableProtein Purification1 Strep-Tactin Superflow cartridge$455
    Strep-tag Starter Kit Cartridge H-PR MacroPrep2-1113-000KitNot ApplicableProtein Purification1 Strep-Tactin Superflow cartridge$455
    Strep/6xHistidine Starter Kit H-PR2-1117-000KitNot ApplicableProtein Purification2X1 ml Columns$395