Contactin-1

Contactins mediate cell surface interactions during the development of the nervous system. Contactin 1 plays a role in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signalling between axons and myelinating glial cells. Contactin 1 also acts as an activating ligand for Notch1, leading to generation of oligodendrocytes. Its interaction with TNR induces neuronal repulsion and an inhibition of neural outgrowth. Contactin 1 shares the L2/HNK carbohydrate epitope with L1, N-CAM, MAG and other cell adhesion molecules from nervous tissue.

Contactin-1 Customer Publications

Images: Loss of TrkB in the cerebellum results in reduction of Contactin-1 localization on Golgi and granule cells. A, B, Loss of TrkB results in reduced localization of Contactin-1 in the IGL of P21 Wnt1::Cre;TrkBfl/fl mice (B) compared with control (A). C, D, Quantification of the area ratio of Contactin-1:vGluT1 (C) and the intensity of Contactin-1 expression (D) expression in control and Wnt1::Cre;TrkB^fl/fl mice. For E–T, yellow represents only colocalized Contactin-1 on individual markers in blue (see Materials and Methods). E–L, Loss of TrkB results in reduced Contactin-1 localization (yellow) on Neurogranin+ Golgi cell axons (blue) and YFP+ granule cell dendrites (blue) in Wnt1::Cre;TrkB^fl/fl; Thy1::YFP mice (F, J) compared with control (E, I). G, H, K, L, Quantification of the area ratio of colocalized Contactin-1:Neurogranin (colocal.:NG) (G), Contactin-1:YFP (colocal.:YFP) (K), and the intensity of colocalized Contactin-1 expression (K, L). M–T, The colocalization of Contactin-1 (yellow) on vGluT+ mossy fibers (blue) and on GFAP+ astroglia is not perturbed in P21 Wnt1::Cre;TrkB^fl/fl mice (N, R) compared with control (M, Q). O, P, S, T, Quantification of the area ratio of colocalized Contactin-1:vGluT1 (colocal.:vGluT1) (O), Contactin-1:GFAP (colocal.:GFAP) (S), and the intensity of colocalized Contactin-1 expression (P, T). Scale bar, 10 μm. U, Immunoblot analysis shows that the expression of Contactin-1 was not perturbed in lysates from four P21 Wnt1::Cre;TrkB^fl/fl mice compared with control. V, Quantification of the intensity of the band for Contactin-1 (135 kDa) normalized to the intensity of tubulin in control (0.94 ± 0.04) and Wnt1::Cre;TrkB^fl/fl lysates (0.90 ± 0.07; p = 0.885).