Caspase 3 & 7 Assay Kit, green

Polycaspase and Caspase in vitro apoptosis kits make it easy to measure cell death and apoptosis in cultured neurons and many other cell types. Just add the reagent to the media, let it incubate 1- 4 hours, wash the cells and read with a fluorescence plate reader, microscope, or flow cytometer.  Apoptotic cells fluoresce red or green.

Comparing Caspase 3&7, Red and Green Kits.

Image: Caspase 3&7 Kit, Green in action. Monocytes of Alzheimer’s disease (AD) patients (left pictures) become apoptotic and therefore cannot properly process amyloid beta. Control monocytes (right) differentiate into macrophages and efficiently phagocytize amyloid beta. Cells were exposed to amyloid beta (5 pg/ml) for 48 hours and then labeled with Polycaspase Assay Kit to stain caspase-3-positive cells green (bottom). In vitro staining with this kit clearly distinguishes apoptotic AD monocytes (bottom left) from healthy control cells (bottom right). Data courtesy of Dr. Milan Fiala, UCLA.