Caspase 9 Assay Kit, red

Polycaspase and Caspase in vitro apoptosis kits make it easy to measure cell death and apoptosis in cultured neurons and many other cell types. Just add the reagent to the media, let it incubate 1- 4 hours, wash the cells and read with a fluorescence plate reader, microscope, or flow cytometer.  Apoptotic cells fluoresce red or green.

Images: Jurkat cells were treated with 1 µM staurosporine to induce caspase 9 activity (left), or a negative control (right) for 3 hours, washed twice, then incubated with ICT’s red caspase 9 inhibitor probe, SR-LEHD-FMK, for 1 hour and examined under a fluorescence microscope (DIC images were also taken). Both induced images reveal several experimental cells which fluoresce red, therefore they have some degree of caspase 9 activity. The non-induced DIC image reveals many control cells, however the corresponding fluorescence image is dark; none of these cells have active caspase 9. Courtesy of Dr. Brian W. Lee, ICT.

FLICA Caspase Detection Kits-FAQs