Flow cytometry and FACS analysis-Human BUCs

Flow cytometry and FACS analysis of P2X3 expression in human BUCs. P2X3 expression in BUCs were compared among in vitro stretched samples and unstretched samples in both normal and interstitial cystitis (IC) patients. A, unstretched normal BUCs. B, stretched normal BUCs. C, unstretched IC BUCs. D, stretched IC BUCs. M1 region represents P2X₃ localization detected by FACS, the percentage of positive P2X3 expression (mean±SE) are 2.3%±0.4%, 9.7%±0.9%, 2.4%±0.4%, 30%±3.0%, respectively. Significant more P2X3 expression in stretched BUCs from IC patients.

Data Courtesy of Dr. Yan Sun University of Maryland School of Medicine Baltimore.