hGDNF Recombinant ProteinCourtesy of Steve Stice et al. Aruna Biomedical and UGA |
Image: Immunofluorescence for STEMEZ(TM) hN2 Human Neurons Discovery Kit differentiated in the presence of 25 ng/ml GDNF. Cells were also counterstained for nuclei (DAPI) and Tuj 1-Neuron-specific class III beta-tubulin and TH-Tyrosine Hydroxylase.
Dopaminergic Neural Differentiation: Human neural progenitor cells were grown on polyornithine/laminin coated 35mm plates (Falcon) for flow cytometry or on polyornithine/laminin coated 4 well slides (Falcon) for immunocytochemistry staining. Cells were plated in proliferation medium. At 24 hours post-plating, the medium was changed to neural differentiation medium (proliferation medium without bFGF) plus or minus 25 ng/ml GDNF (Neuromics). Medium were changed every three days. Cells were harvested at differentiation day 0, 3, 7, 14 and 21 for further analysis.