VR1-N (TRPV1-N)

Images courtesy of Dr. Hui-lin Pan, Penn State; Dr. Igor Spigelman, UCLA; Dr. Norman Capra, Univ. of Maryland; and Dr. Janet Keast, University of New South Wales.

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Cultures were incubated overnightat 4◦C with chicken anti-CGRP antibodies (1:500 in PBS; Neuromics,Edina, MN) or rabbit anti-TRPV1 antibodies (1:1000 in PBS; Neuromics). Immunoreactive proteins were detected following incubation with Rhodamine Red X-conjugated donkey anti-chicken or FITC-conjugated donkey anti-rabbit IgG polyclonal antibodies (1:100 in PBS, Jackson Immuno-Research Laboratories, West Grove, PA) for 1 h at room temperature. Prior to viewing, cells were mounted using Vectashield mounting media (Vector Laboratories, Burlingame, CA) containing 4',6 diamidino-2-phenylindole (DAPI) to identify nuclei. Cells were photographed with an Olympus DP70 camera mounted on an Olympus BX41 fluorescent microscope and image collection and analysis performed using Olympus MicroSuite Five image processing software (Center Valley, PA).

Image: CGRP and TRPV1 are co-expressed in most cultured trigeminal ganglion neurons. (A and C) CGRP expression in d2 trigeminal ganglion neurons plated on poly-d-lysine-coated coverslips. (B and D) The same culture shown in panel A or C costained for TRPV1 expression. Magnification bar = 200m (A and B) or 40m (C and D). Journal of Ethnopharmacology 115 (2008) 238–248.

 

VR1 staining in rat dorsal root ganglion. Image courtesy Dr. Janet Keast, University of New South Wales.
VR1 staining in rat dorsal root ganglion. Image courtesy Dr. Janet Keast, University of New South Wales.
VR1-N staining in rat trigeminal ganglion. Image courtesy of Dr. Norman Capra, University of Maryland
VR1-N staining in rat trigeminal ganglion. Image courtesy of Dr. Norman Capra, University of Maryland