Keiichi Hiramoto1,and Emiko Kasahara. Long-term UVA eye irradiation causes decreased learning ability in mice. Photodermatology, Photoimmunology & Photomedicine. Accepted Online. DOI: 10.1111/phpp.12229

... blocked with 5% skim milk overnight at 4°C. After blocking, the membranes were incubated at 25°C for 1 hour with primary antibodies against β-secretase (BACE1,1:1000; Neuromics, Edina, MN), γ-secretase (APHC-1A, 1:1000 ...