A.P. Shah, F.R. Carreno, H. Wu, Y.A. Chung, A. Frazer. Role of TrkB in the anxiolytic-like and antidepressant-like effects of vagal nerve stimulation: Comparison with desipramine. Neuroscience. Available online 16 February 2016. doi:10.1016/j.neuroscience.2016.02.024

... at 100 V for 1 h. Membranes were incubated overnight at 4 °C using anti-pY705 TrkB (1:1000 in 2.5% bovine serum albumin (BSA) in Tris-buffered saline with 0.1% Tween 20 (TBST), Abcam,USA) or anti-TrkB (full length, 1:5000 in 2.5% milk in TBST, Neuromics, USA). ...

Laura L. Susick, Alexandria C. Chrumka, Steven M. Hool, Alana C. Conti. Dysregulation of TrkB Phosphorylation and proBDNF protein in Adenylyl Cyclase 1 and 8 Knockout Mice in a Model of Fetal Alcohol Spectrum Disorder. Alcohol: An International Biomedical Journal. DOI: http://dx.doi.org/10.1016/j.alcohol.2015.11.008 

...TrkB (GT15080; Neuromics, Edina, MN)...

Albert Giralt, Renata Coura and Jean-Antoine Girault. Pyk2 is essential for astrocytes mobility following brain lesion. Article first published online: 11 DEC 2015 DOI: 10.1002/glia.22952

... goat polyclonal antibodies for TrkB (1:1,000, GT15080,Neuromics, Edina, ...

F R Carreno, J J Donegan, A M Boley, A Shah, M DeGuzman, A Frazer and D J Lodge. Activation of a ventral hippocampus-medial prefrontal cortex pathway is both necessary and sufficient for an antidepressant response to ketamine. Molecular Psychiatry , (1 December 2015) | doi:10.1038/mp.2015.176

... antibody was stripped out with stripping buffer (Thermo Scientific, Rockford, IL, USA), and the membranes were cut and re-probed with anti-actin peroxidase conjugated (1:200 000, Sigma, A3854/Lot 062M4795V) and goat anti-full length TrkB (1:5000, Neuromics, Edina, MN [GT15080] ...

Benmansour S., Adeniji O.S., Privratsky A.A, & Frazer A. (2015) Effects of Long-Term Treatment with Estradiol and Estrogenreceptor Subtype Agonists on Serotonergic Function in Ovariectomized Rats. Neuroendocrinology, Available Online 7/1/2015 ISSN: 0028-3835 (Print) eISSN: 1423-0194 (Online) DOI:10.1159/000437268

...phospho-Y816 (1:4000), anti-TrkB (1:10,000, Neuromics, Edina, MN), and the following primary
antibodies (from Cell Signaling Technology, Danvers, MA) all used at 1:1000: anti- ...

Carlos B. Mantilla,Sarah M. Greising,Jessica M. Stowe,Wen-Zhi Zhan,Gary C. Sieck. TrkB kinase activity is critical for recovery of respiratory function after cervical spinal cord hemisection. DOI: 10.1016/j.expneurol.2014.05.02.

...TrkB protein was immunoprecipitated using anti-TrkB (Neuromics # GT15080). Following electrophoresis, protein was transferred to a PVDF membrane and probed using 1:500 phosphorylated TrkB (Cell Signaling #4621S) and 1:1000 TrkB (Neuromics #GT15080)...

Pawan K. Vohra, Michael A. Thompson, Venkatachalem Sathish, Alexander Kiel, Calvin Jerde, Christina M. Pabelick, Brij B. Singh, Y.S. Prakash, TRPC3 Regulates Release of Brain-Derived Neurotrophic Factor From Human Airway Smooth Muscle. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Available online 27 July 2013, ISSN 0167-4889, http://dx.doi.org/10.1016/j.bbamcr.2013.07.019

...samples were subjected to SDS-PAGE, PVDF membrane transfer and immunoblotted against TrkB antibody (Neuromics, GT15080)...

Y. S. Prakash, Michael A. Thompson, and Christina M. Pabelick "Brain-Derived Neurotrophic Factor in TNF-α Modulation of Ca2+ in Human Airway Smooth Muscle", American Journal of Respiratory Cell and Molecular Biology, Vol. 41, No. 5 (2009), pp. 603-611. doi: 10.1165/rcmb.2008-0151OC.

...Western Blotting:The supernatants were denatured at 100°C for 3 to 5 minutes and 45 μl of sample subjected to SDS-PAGE using the Criterion Gel System (Bio-Rad) and a 4–15% gradient gel. The gels were run at a current of 200 V for 60 minutes followed by transfer to PVDF membranes (Bio-Rad). Membranes were blocked for 1 hour in 5% nonfat dry milk in Tris-buffered saline containing 0.1% Tween 20, followed by incubation with goat polyclonal anti-TrkB (1:1,000 dilution; Neuromics, Minneapolis, MN) or mouse monoclonal anti-p75NTR (1:100 dilution) antibody overnight, and horseradish peroxidase–conjugated secondary antibody for 2 hours. Blots were then visualized by exposure to BioMax XAR film (Eastman Kodak Co., Rochester, NY) using Supersignal West Dura substrate (Pierce Biotechnology, Rockford, IL)...

Havan Furmaga, Flavia Regina Carreno, and Alan Frazer. Vagal Nerve Stimulation Rapidly Activates Brain-Derived Neurotrophic Factor Receptor TrkB in Rat Brain. PLoS One. 2012; 7(5): e34844. Published online 2012 May 1. doi: 10.1371/journal.pone.0034844

...Rats were sacrificed by rapid decapitation, brains quickly removed and hippocampi were dissected out on an ice-cooled dish. Samples were stored at −80°C until processed for Western blot analysis. Samples were homogenized in a lysis buffer (50 mM Tris, 1 mM EDTA, 0.35% NA Deoxycholate, 150 mM NaCl, 1% Igepal, H2O, and 10 µl of protease inhibitor mixture (Sigma) per 100 mg tissue), incubated on ice for 30 min and then centrifuged (13000 g, 15 min). Protein levels of the collected supernates were measured using the Bradford assay (Biorad, Hercules, CA). Proteins were separated in a SDS-PAGE gel and blotted onto a nitrocellulose membrane. Membranes were incubated at 4°C overnight with the anti-TrkB (110000 in 1% BSA in TBST, Neuromics, Edina, MN)...Equal loading was confirmed using anti-β-actin (1100,000 in 1% BSA in TBST, Sigma), which was routinely used for normalization. Membranes were washed with TBS/0.1% Tween (TBST) and incubated with horseradish peroxidase conjugated secondary antibody (15000 in 1% BSA in TBST, Sigma). Secondary antibodies were visualized using enhanced chemiluminescence kits (Pierce, Rockford, IL) followed by an exposure to X-ray film for detection.Results were calculated as the ratio of phosphorylated TrkB at specific tyrosines to total TrkB...

F. R. Carreño, J. D. Walch, M. Dutta, T. P. Nedungadi, J. T. Cunningham. Brain-Derived Neurotrophic Factor-Tyrosine Kinase B Pathway Mediates NMDA Receptor NR2B Subunit Phosphorylation in the Supraoptic Nuclei Following Progressive Dehydration. Journal of Neuroendocrinology. Volume 23, Issue 10, pages 894–905, October 2011. DOI: 10.1111/j.1365-2826.2011.02209.x

...TrkB receptor (Neuromics, Edina, MN, USA)...

Anantha Vijay R. Santhanam PhD, Leslie A. Smith, and Zvonimir S. Katusic MD, PhD. Brain-Derived Neurotrophic Factor Stimulates Production of Prostacyclin in Cerebral Arteries. Stroke. 2009 Published online before print December 17, 2009, doi: 10.1161/STROKEAHA.109.564492

Y.S. Prakash, Michael A Thompson, and Christina M Pabelick. Brain Derived Neurotrophic Factor in TNF Modulation of Ca2+ in Human Airway Smooth Muscle. Am. J. Respir. Cell Mol. Biol., Feb 2009; doi:10.1165/rcmb.2008-0151OC.

... goat polyclonal anti-TrkB (Neuromics, Minneapolis, MN; 1:1000 dilution)...

Guo W, Robbins MT, Wei F, Zou S, Dubner R, Ren K (2006). Supraspinal Brain-Derived Neurotrophic Factor Signaling: A Novel Mechanism for Descending Pain Facilitation. J Neurosci. 26(1):126-137. The Journal of Neuroscience, January 4, 2006, 26(1):126-137

...with mouse anti-TrkB (1:600; Neuromics, Bloomington, MN)...