Han Pin Pui and Yumiko Saga. Gonocytes-to-spermatogonia transition initiates prior to birth in murine testes and it requires FGF signaling. Mechanisms of Development. Volume 144, Part B, April 2017, Pages 125–139. http://dx.doi.org/10.1016/j.mod.2017.03.002

...Immunofluorescence & in situ hybridization

Whole testes or testicular explants (for culture experiments) were fixed in 4% paraformaldehyde (Nakalai)/PBS at 4 °C overnight. Then the specimens were equilibrated in sterile 30% sucrose/PBS, embedded in 22-oxacalcitriol (OCT) compound (Sakura Finetek) and sectioned to 6 μm thickness. For immunofluorescent staining, sections were pre-treated with Antigen Unmasking Solution (Vector Laboratories) or Target Retrieval Solution (Dako) at 105 °C for 5 min. This was followed by blocking with 3% skimmed milk/PBST (0.1% Tween 20/PBS) for 1 h at room temperature and incubated with primary antibodies in Can Get Signal A buffer (TOYOBO) at 4 °C overnight. The antibodies used and their dilutions were goat anti-GFRA1 (1:500, Neuromics)goat anti-RET (1:500, Neuromics), rabbit anti-PLZF (1:500, Santa Cruz Biotechnology), rabbit anti-phosphorylated Histone 3 (1:500, Cell Signaling Technology), mouse anti-phosphorylated Histone 3 (1:20, gift from Hiroshi Kimura (Hayashi-Takanaka et al., 2009)), rabbit anti-phosphorylated ERK1/2 (1:500, Cell Signaling Technology), rabbit anti-phosphorylated S6 (1:500, Cell Signaling Technology), rabbit anti-FOXO1 (1:100, Cell Signaling Technology), goat anti-E-Cadherin (1:1000, R&D Systems), rabbit anti-Laminin (1:1000, Sigma-Aldrich), rat anti-Laminin (1:100, MP Biomedicals), rabbit anti-MVH (1:500, Abcam) and rat anti-TRA98 (1:500, gift from Yoshitake Nishimune). After rinsing, the sections were incubated with appropriate secondary antibody conjugated with Alexa488 or Alexa594 or Alexa647 (1:500, Life Technologies) or CY5 (1:500, Rockland Immunochemicals) in PBST at 4 °C overnight....

Ryo Hotta, Lily Cheng, Hannah K. Graham, Nandor Nagy, Jaime Belkind-Gerson, George Mattheolabakis, Mansoor M. Amiji, Allan M. Goldstein. Delivery of enteric neural progenitors with 5-HT4 agonist-loaded nanoparticles and thermosensitive hydrogel enhances cell proliferation and differentiation following transplantation in vivo. Biomaterials, 2016

... Primary antibodies included goat anti-Ret (1:20, Neuromics, Edina, MN), mouse anti-Tuj1 (1:100, Covance, Dedham, MA), mouse anti-Tuj1 conjugated Alexa Flour 488 (1:400, BioLegend, Dedham, MA), rabbit anti-calretinin (1:200, Life Technologies, Carlsbad, CA), rabbit anti ...

Athanasia Stathopoulou, Dipa Natarajan, Pinelopi Nikolopoulou, Alexandra L. Patmanidi, Zoi Lygerou, Vassilis Pachnis, Stavros Taraviras. Inactivation of Geminin in neural crest cells affects the generation and maintenance of enteric progenitor cells, leading to enteric aganglionosis. Developmental Biology Available online 3 December 2015. doi:10.1016/j.ydbio.2015.11.023

... goat anti-Ret (1:300, Neuromics)...

Ellen Merrick Schilla, Jonathan I. Lake, Olga A. Tusheva, Nandor Nagy, Saya K. Bery, Lynne Foster, Marina Avetisyan, Stephen L. Johnson, William F. Stenson, Allan M. Goldstein, Robert O. Heuckeroth. Ibuprofen slows migration and inhibits bowel colonization by enteric nervous system precursors in zebrafish, chick and mouse. Developmental Biology. Received 28 July 2015, Revised 31 August 2015, Accepted 7 September 2015, Available online 14 November 2015. doi:10.1016/j.ydbio.2015.09.023

....Goat anti-RET, 1:100, GT15002, Neuromics; ...

Marina Avetisyan, Hongtao Wang, Ellen Merrick Schill, Saya Bery, John R. Grider, John A. Hassell, Thaddeus Stappenbeck, and Robert O. Heuckeroth. Hepatocyte Growth Factor and MET Support Mouse Enteric Nervous System Development, the Peristaltic Response, and Intestinal Epithelial Proliferation in Response to Injury. The Journal of Neuroscience, 19 August 2015, 35(33): 11543-11558; doi: 10.1523/JNEUROSCI.5267-14.2015

...PGP9.5 (guinea pig, 1:100; #GP14104, Neuromics), RET (goat, 1:800; #GT15002, 1:800, Neuromics)...

Dario Bonanomi, Onanong Chivatakarn, Ge Bai, Houari Abdesselem, Karen Lettieri, Till Marquardt, Brian A. Pierchala, Samuel L. Pfaff. Ret Is a Multifunctional Coreceptor that Integrates Diffusible- and Contact-Axon Guidance Signals. Cell, Volume 148, Issue 3, 568-582, 3 February 2012. Copyright © 2012 Elsevier Inc. All rights reserved. 10.1016/j.cell.2012.01.024.

...goat anti-Ret (Neuromics, extracellular epitope, 1:300)...

Danny Galleguillos, José A. Fuentealba1, Luis M. Gómez1, Mathias Saver, Andrea Gómez, Kevin Nash, Corinna Burger, Katia Gysling, María E. Andrés. Nurr1 regulates RET expression in dopamine neurons of adult rat midbrain. Journal of Neurochemistry. Volume 114, Issue 4, pages 1158–1167, August 2010.

...anti-RET 1 : 5000, Neuromics GT15002...

Ming Fu, Yoshiharu Sato, Ariel Lyons-Warren, Bin Zhang, Maureen A. Kane, Joseph L. Napoli and Robert O. Heuckeroth. Vitamin A facilitates enteric nervous system precursor migration by reducing Pten accumulation. Development 137, 631-640 (2010) doi:10.1242/dev.040550.

...Ret (goat; Neuromics, 1:100)...

Nina Bergelin, Christoffer Löf, Sonja Balthasar, Veronica Kalhori, and Kid Törnquist. S1P1, and VEGFR-2 Form a Signaling Complex with Extracellularly Regulated Kinase 1/2 and Protein Kinase C-alpha Regulating ML-1 Thyroid Carcinoma Cell Migration. This version published online on May 25, 2010. Endocrinology, doi:10.1210/en.2009-1387

...conjugated goat antirabbit from Bio-Rad Laboratories (Hercules, CA). Rearranged in transformation (RET) antibody was from Neuromics (Edina, MN). Secondary antibodies (Alexa Fluor goat antirabbit 568 and goat antimouse 488) for immunocytochemistry wereobtained from MolecularProbes... 

Ming Fu, Yoshiharu Sato, Ariel Lyons-Warren, Bin Zhang, Mureen A. Kane, Joseph L. Napoli, and Robert O. Heuckeroth Vitamin A facilitates enteric nervous system precursor migration by reducing Pten accumulation. Development, Feb 2010; 137: 631 - 640.

... Ret (goat; Neuromics, 1:100)...

Gaurav Tyagi, Kay Carnes, Carla Morrow, Natalia V. Kostereva, Gail C. Ekman, Daryl D. Meling, Chris Hostetler, Michael Griswold, Kenneth M. Murphy, Rex A. Hess, Marie-Claude Hofmann and Paul S. Cooke. Loss of Etv5 Decreases Proliferation and RET Levels in neonatal Mouse Testicular Germ Cells and Causes an Abnormal First Wave of Spermatogenesis. DOI:10.1095/biolreprod.108.075200

...For RET immunohistochemistry the slides were washed with PBS and incubated for 30 min in 10% (v/v) normal rabbit serum. After serum block, the sections were incubated for 1.5 h with anti-RET (Neuromics,.Bloomington, MN; 1:100, goat polyclonal)...

Thomas W. Gould, Shigenobu Yonemura, Ronald W. Oppenheim, Shiho Ohmori, and Hideki Enomoto.  The Neurotrophic Effects of Glial Cell Line-Derived Neurotrophic Factor on Spinal Motoneurons Are Restricted to Fusimotor Subtypes. The Journal of Neuroscience, February 27, 2008, 28(9):2131-2146

...mouse Ret (Neuromics, Edina, MN), human Ret (Neuromics), and activated caspase-3 (Cell Signaling, Danvers, MA). Muscles or sections were rinsed and then......

Edgar R Kramer, Liviu Aron,1 Geert M. J Ramakers, Sabine Seitz, Xiaoxi Zhuang, Klaus Beyer, Marten P Smidt, and Rüdiger Klein1. Absence of Ret Signaling in Mice Causes Progressive and Late Degeneration of the Nigrostriatal System PLoS Biol. 2007 March; 5(3): e39.

...Primary antibodies used were goat anti-Ret (1:25; Neuromics, Edina, Minnesota, United States)...

... goat anti-Ret antibody (Neuromics,. Inc., Minneapolis, MN, 1:200)...