Ganesan Ramesh, Arthur Berg, and Calpurnia Jayakumar. Plasma netrin-1 is a diagnostic biomarker of human cancers. Biomarkers. Author manuscript; available in PMC 2011 July 26. Published in final edited form as: Biomarkers. 2011 March; 16(2): 172–180. Published online 2011 February 8. doi: 10.3109/1354750X.2010.541564.

...Stage I–III renal cell carcinoma and normal tissue section (Tissue Array) was obtained from Biomax to immunolocalize netrin-1, as described previously (29). Briefly, tissue sections were dewaxed and rehydrated with graded ethanol (100%, 90%, 70% and 30%) and then washed with PBS. Antigen retrieval was carried out using citrate buffer and steamer. The tissue section was permeabilized with 0.2% Triton X-100 in PBS, and washed and blocked with PBS containing 5% donkey serum and 1% BSA. Primary antibodies included a chicken anti-netrin-1 polyclonal antibody (Neuromics cat # CH23002). Primary antibodies were detected using secondary antibodies conjugated with biotin, which was followed by incubation with streptavidin-horseradish peroxidase (Pierce). Slides were mounted in permount and photographed using an Olympus microscope attached to a CCD camera...

Amelia Stanco, Charles Szekeres, Nikhil Patel, Sarada Rao, Kenneth Campbell, Jordan A. Kreidberg, Franck Polleux, and E. S. Anton. Netrin-1–3β1 integrin interactions regulate the migration of interneurons through the cortical marginal zone. PNAS, Apr 2009; doi:10.1073/pnas.0811343106

...netrin-1 polyclonal antibodies (Neuromics)...

Weiwei Wang, W. Brian Reeves, and Ganesan Ramesh. Netrin-1 increases proliferation and migration of renal proximal tubular epithelial cells via the UNC5B receptor. Am J Physiol Renal Physiol, Feb 2009; doi:10.1152/ajprenal.90686.2008.

...non-specific sites were blocked with 5% normal goat serum/1% BSA in PBS and incubated with chicken anti-netrin-1 antibody (Neuromics) overnight at 40 C. After washing, cells were incubated with FITC-conjugated goat anti-chicken antibody for 1 hour...

W.Brian Reeves, Osun Kwon, and Ganesan Ramesh. Netrin-1 is an early biomarker of acute kidney injury. Am J Physiol Renal Physiol (January 30, 2008). doi:10.1152/ajprenal.00507.2007.

...incubated with the primary chicken anti-netrin-1 polyclonal antibody (Neuromics cat#CH23002). at a 1:100 dilution for 18hours...

Weiwei Wang, W.Brian Reeves, and Ganesan Ramesh1. Part 1: Functional Studies Netrin-1 protects against ischemia reperfusion injury of the kidney. Am J Physiol Renal Physiol (January 23, 2008). doi:10.1152/ajprenal.00508.2007.

...chicken anti-netrin-1 polyclonal antibody (Neuromics cat#CH23002)...