Yoritsuna Yamamoto, Masanori Fujita, Yuji Tanaka, Itaru Kojima, Yasuhiro Kanatani, Masayuki Ishihara, and Shoichi Tachibana. Low Oxygen Tension Enhances Proliferation and Maintains Stemness of Adipose Tissue–Derived Stromal Cells. BioResearch Open Access Volume 2, Number 3, June 2013 Mary Ann Liebert, Inc. DOI: 10.1089/biores.2013.0004

...ASCs were cultured on noncoated glass coverslips at a density of 2 · 104/mL. Cells were fixed with 10% neutral buffered formalin, treated with 0.1% (v/v) Triton X-100 in PBS for 10 min and incubated sequentially with Block Ace. They were incubated with monoclonal mouse Oct3/4 antibody (Neuromics) diluted at 1:200 for 1 h at room temperature. After washing, they were incubated with Alexa Fluor 488 donkey anti-goat IgG (H + L) conjugate (1:200; Life Technologies Corporation) for 1 h and then 4-diamino-2-phenylindoke (DAPI; Pierce). Immunofluorescence images were recorded with an Axio Imager A2 microscope (Carl Zeiss Microscopy) equipped with an AxioCam cooled-CCD camera system (Carl Zeiss Microscopy), which was operated with AxioVision software (Carl Zeiss Microscopy)...

Hisako Muramatsu, Taiki Kusano, Masahiro Sato, Yoshihiro Oda, Kyoko Kobori and Takashi Muramatsu.  Embryonic stem cells deficient in I β1,6-N-acetylglucosaminyltransferase exhibit reduced expression of embryoglycan and the loss of a Lewis X antigen, 4C9. Glycobiology 2008 18(3):242-249; doi:10.1093/glycob/cwm138.

...ES cells were treated with trypsin/EDTA for 5 min and were plated onto 0.1% gelatin-coated dishes at 37°C for 30 min, and unadhered ES cells were collected and cultured in the ES medium in 2-well chamber slides (Nalge Nunc International, Naperville, IL) coated with 0.5% gelatin overnight. Cells were fixed with 4% paraformaldehyde for 20 min at room temperature. Fixed cells were blocked at room temperature for 45 min with 10% normal goat serum/1% BSA/0.1% Triton X-100/PBS for Oct 3/4 (Neuromics)...

Jan Pruszak, Kai Christian Sonntag, Moe Hein Aung, Rosario Sanchez-Pernaute and Ole Isacson. Markers and Methods for Cell Sorting of Human Embryonic Stem Cell-derived Neural Cell Populations. Stem Cells published online Jun 21, 2007.

...IgG1 anti-Nestin (1:1000), goat polyclonal anti-Otx2 (1:1000), goat anti-Oct-3/-4 (1:1000; all from Edina, MN)....