Abdellatif Benraiss,Su Wang,Stephanie Herrlinger, Xiaojie Li, Devin Chandler-Militello, Joseph Mauceri, Hayley B. Burm, Michael Toner, Mikhail Osipovitch,Qiwu Jim Xu, Fengfei Ding, Fushun Wang,Ning Kang,Jian Kang, Paul C. Curtin, Daniela Brunner, Martha S. Windrem, Ignacio Munoz-Sanjuan, Maiken Nedergaard & Steven A. Goldman. Human glia can both induce and rescue aspects of disease phenotype in Huntington disease. Nature Communications 7, Article number: 11758 doi:10.1038/ncomms11758 Published 07 June 2016

...Histology
Animals were killed using sodium pentobarbital and perfused transcardially with saline followed by 4% paraformaldehyde, and their brains were processed for immunocytochemistry as previously described. Sagittal equidistant cryosections of sections (20 μm) spanning the whole brain were analysed. Human cells were identified through immunostaining with anti-human nuclear antigen (1:800, MAB1281, Millipore, Temecula, CA, USA). Engrafted human cells were mapped using Metamorph imaging software and an automated fluorescence microscope (Leica Microsystems, Wetzlar, Germany). Brain sections were then co-stained to define phenotype, using combinations of the following antibodies: mouse anti-huntingtin antibody clone EM48 (1:250, MAB5374, Millipore); mouse anti-glial fibrillary acidic protein (GFAP) (1:800, SMI-21, Covance, Princeton, NJ, USA); rabbit anti-Olig2 (1:400, RA25081 Neuromics, Edina, MN, USA); rabbit anti-PDGFRα (1:400, 5241 Cell Signaling Technology, Danvers, MA, USA). Slides were analysed serially every twenty-fourth section using the optical fractionator method to estimate the total number of engrafted human cells of each histological marker (GFAP, Olig2, PDGFRα) using StereoInvestigator imaging software (MicroBrightField, Burlington, VT, USA)...

Martha S. Windrem, Steven J. Schanz, Carolyn Morrow, Jared Munir, Devin Chandler-Militello, Su Wang, and Steven A. Goldman. A Competitive Advantage by Neonatally Engrafted Human Glial Progenitors Yields Mice Whose Brains Are Chimeric for Human Glia. The Journal of Neuroscience, 26 November 2014, 34(48): 16153-16161; doi: 10.1523/JNEUROSCI.1510-14.2014

...Olig2 Rabbit anti-Olig2 1:500 RA25017 Neuromics...

Crystal R. McClain, Fraser J. Sim and Steven A. Goldman. Pleiotrophin Suppression of Receptor Protein Tyrosine Phosphatase-β/ζ Maintains the Self-Renewal Competence of Fetal Human Oligodendrocyte Progenitor Cells. The Journal of Neuroscience, 24 October 2012, 32(43): 15066-15075; doi: 10.1523/​JNEUROSCI.1320-12.2012.

...Olig2 (1:1000, Neuromics)...

Janne Hakanena, Sébastien Duprat and Marjo Salminena. Netrin1 is required for neural and glial precursor migrations into the olfactory bulb. Developmental Biology Volume 355, Issue 1, 1 July 2011, Pages 101-114.

...OLIG2 1:800 (Neuromics)...

P.G. Franco, L. Silvestroff, E.F. Soto and J.M. Pasquini. Thyroid hormones promote differentiation of oligodendrocyte progenitor cells and improve remyelination after cuprizone-induced demyelination. doi:10.1016/j.expneurol.2008.04.039 

...Olig 1-2-3 antibodies were from Neuromics Antibodies (Edina, MN); ...