Sarah Krupp. Effects of neonatal capsaicin on formalin-induced ATF3 expression in the adult rat. University of Louisville. ThinkIR: The University of Louisville's Institutional Repository Electronic Theses and Dissertations. 8-2016
....Animal Perfusion and Tissue Processing:
Upon completion of all experiments, two days post Gformalin injections, animals were sacrificed with with an IP injection of 10 cc pentobarbital and transcardially perfused with paraformaldehyde (PFA) for tissue fixation as previously described (Xu)et al.,)2014). The L4 and L5 DRG were immediately dissected out and placed into 4% PFA for overnight fixation and transferred to 30% sucrose for a minimum of 4 days for cryoprotection. Tissue was then embedded using freezing medium and cut into 14μm sections for further analysis.
L4GL5 DRG were stained for TRPV1, TRPA1, and ATF3 using the following antibodies: guinea pig anti-TRPV1 (1:1000,) Neuromics) GP14100) with donkey anti-guinea pig 405 (1:100 Jackson Immuno)706475148), rabbit anti-TRPA1 (1:80,000,) Neuromics RA14135) with donkey anti-rabbit 488 (1:100) Jackson Immuno 711545152), and mouse anti-ATF3 (1:250) Abcam, ab58668) with donkey anti-mouse Cy3 (1:100) Jackson Immuno 715165151) preadsorbed with 20 µl rat serum and 80 µl blocking serum to prevent non-specific binding.....
Yun Sook Kim, PhD, Hoon Kap Jung, DDS, Tae Kyung Kwon, DDS, Chin Soo Kim, DDS, PhD, Jin Hyun Cho, DDS, PhD, Dong Kuk Ahn, DDS, PhD, Yong Chul Bae, DDS, PhD.Expression of Transient Receptor Potential Ankyrin in Human Dental Pulp. Journal of Endodontics. Available online 8 June 2012. doi.org/10.1016/j.joen.2012.04.024.
... rabbit anti-TRPA1 antiserum (1:1,000, RA14135; Neuromics, Edina, MN)...
Jin Y. Ro, Jong-Seok Lee and Youping Zhang. Activation of TRPV1 and TRPA1 leads to muscle nociception and mechanical hyperalgesia. doi:10.1016/j.pain.2009.04.021.
... TRPA1 (1:1500; rat polyclonal, Neuromics)...