gp130 or CD130

Manfred Andratsch, Norbert Mair, Cristina E. Constantin, Nadja Scherbakov, Camilla Benetti, Serena Quarta, Christian Vogl, Claudia A. Sailer, Nurcan Üceyler, Johannes Brockhaus, Rudolf Martini, Claudia Sommer, Hanns Ulrich Zeilhofer, Werner Müller, Rohini Kuner, John B. Davis, Stefan Rose-John, and Michaela Kress. A Key Role for gp130 Expressed on Peripheral Sensory Nerves in Pathological Pain. The Journal of Neuroscience, October 28, 2009, 29(43):13473-13483; doi:10.1523/JNEUROSCI.1822-09.2009

 ...DRGs were washed with PBS and fixed with 4% PFA for 20 min. After 3 x 10 min washing with PBS, ganglia were frozen in methylbutan. Sections (12 µm) were cut with a Leica CM 1850 Cryomicrotome, permeabilized with 0.1% TX-100, and blocked with blocking buffer. Sections were incubated with primary antibodies overnight at +4°C in a wet chamber. After washing in PBS, sections were incubated with the secondary antibodies, and embedded in Mowiol (Calbiochem). Indirect immune fluorescence was detected with a Zeiss Axiovert 200M microscope and analyzed with the MetaMorph Imaging Software Series 7.1 (Molecular Devices, Visitron Systems). The following antibodies were used: anti-gp130 (1:50, extracellular epitope, Neuromics); anti-Nav1.8 (1:200, was a generous gift from Prof. Dr. H. G. Knaus, Innsbruck, Austria) (see also supplemental Fig. 2, available at www.jneurosci.org as supplemental material) (Liu et al., 2006); anti-CGRP (1:300, ImmunoStar), anti-Isolectin IB4 (1:1000, Invitrogen); Alexa Fluor 488 goat anti-rabbit IgG (1:1000, H+L), Alexa Fluor 594, donkey anti-goat IgG (1:1000, all Invitrogen)...