Primary Neurons and Astrocytes -Primary human, rat and mouse neurons and astrocytes by Category
Geoffrey E. Bray, Zhengxin Ying, Landon D. Baillie, Ruiling Zhai, Sean J. Mulligan, and Valerie M.K. Verge. Extracellular pH and Neuronal Depolarization Serve as Dynamic Switches to Rapidly Mobilize trkA to the Membrane of Adult Sensory Neurons. The Journal of Neuroscience, 8 May 2013, 33(19):8202-8215; doi:10.1523/JNEUROSCI.4408-12.2013.
...anti-phospho-trkA (1:100; Neuromics) diluted in blocking solution, and incubated at room temperature (trkA) or 4OC...
Yuan Lu, Yuanyuan Ji, Sundar Ganesan, Robert Schloesser, Keri Martinowich, Mu Sun, Fan Mei, Moses V. Chao, and Bai Lu. TrkB as a Potential Synaptic and Behavioral Tag. The Journal of Neuroscience, 17 August 2011, 31(33): 11762-11771; doi: 10.1523/JNEUROSCI.2707-11.2011.
...Slices were taken out of the recording chamber at different time points after stimulation. CA1 area was quickly dissected on ice and stored on dry ice. Four pieces of CA1 for each condition were lysed in an ice-cold RIPA lysis buffer. The lysates were mixed with sample buffer for SDS-PAGE. Samples were boiled for 5 min, subjected to SDS-PAGE, and immunoblotted using rabbit anti-Phospho-TrkA (Tyr490) (1:1000, Neuromics) and rabbit anti-TrkB [1:1000; TrkB (794): sc-12; Santa Cruz Biotechnology]. Densitometric analysis was conducted using Molecular Analysis software of Bio-Rad. The same experiments (with multiple time points) were repeated at least six times (n = 6)...