Xiuhua Gao, Yonghua Li, Hongxia Wang, Chuanbao Li and Jianguang Ding. Inhibition of HIF-1α decreases expression of pro-inflammatory IL-6 and TNF-α in diabetic retinopathy. Acta Ophthalmologica. doi: 10.1111/aos.13096
... a polyvinylidene fluoride membrane. Membranes were incubated with the rabbit anti-IL-1R, anti-IL-6R, anti-TNFR1 and anti-Caspase-3 primary antibodies (1:500,obtained from Neuromics and Abcam Co). After being fully washed ...
Yang J, He F, Meng Q, Sun Y, Wang W, Wang C. Inhibiting HIF-1α Decreases Expression of TNF-α and Caspase-3 in Specific Brain Regions Exposed Kainic Acid-Induced Status Epilepticus. Cell Physiol Biochem 2016;38:75-82
....Western Blot Analysis: The brain tissues were removed, homogenized, centrifuged and incubated with streptavidin beads. The beads were washed and precipitated by centrifugation and sample buffer was added to the collected beads. Beads were pelleted again by centrifugation to obtain supernatant. The supernatant was then diluted to the same volume and applied to SDS-PAGE. Membranes were incubated with the rabbit anti-Caspase-3 primary antibody (1:500, Neuromics) and goat anti-rabbit secondary antibody (1:500). Immunoreactive proteins were detected by enhanced chemiluminescence. The membrane was also processed to detect β-actin for equal loading. The densities of protein bands were analyzed using the Scion image software...
Maia Cabrera , Natalia Gomez, Federico Remes Lenicov, Emiliana Echeverría, Carina Shayo, Albertina Moglioni, Natalia Fernández, Carlos Davio. G2/M Cell Cycle Arrest and Tumor Selective Apoptosis of Acute Leukemia Cells by a Promising Benzophenone Thiosemicarbazone Compound. Published: September 11, 2015DOI: 10.1371/journal.pone.0136878
...anti-caspase 3 antibody from Neuromics (Edina, MN, United States). A horseradish peroxidase-conjugated goat anti-mouse and anti-rabbit were used as the secondary antibody... Western Blot Protocol: Cells were washed in PBS and lysed in 50 mM Tris–HCl pH 6.8, 2% SDS, 100 mM 2-mercaptoethanol, 10% glycerol and 0.05% bromophenol blue and sonicated to shear DNA. Cellular proteins from total cell lysates (20 μg) were electrophoresed on 8–15% SDS polyacrylamide gel and transferred to nitrocellulose membranes. Blots were blocked with 5% non-fat powdered milk in TBS containing 0.05% Tween-20 and probed with the indicated primary antibodies followed by horseradish-peroxidase-conjugated secondary antibodies. Reactivity was developed by enhanced chemiluminescence (ECL) according the manufacturer’s instructions (Amersham Life Science, England)..
Ramaswamy Sharma and Alexander Gow. Minimal role for caspase 12 in the unfolded protein response in oligodendrocytes in vivo. Journal of Neurochemistry, 2007, 101, 889–897 doi:10.1111/j.1471-4159.2007.04541.x.
... Rabbit anti-cleaved caspase 3 (0.3 lg/mL; Neuromics, Northfield, MN, USA)...