T.A. Retson, E.J. Van Bockstaele. Coordinate regulation of noradrenergic and serotonergic brain regions by amygdalar neurons. Journal of Chemical Neuroanatomy. Available online 4 May 2013. doi.org/10.1016/j.jchemneu.2013.04.003,

...guinea pig antibody directed against pro-dynorphin (ppDYN; Neuromics, Inc., Minneapolis, MN) at 1:2000...

M. Luisa Laorden, Szilamér Ferenczi, Bernadett Pintér-Kübler, Laura L. González-Martín, M. Carmen Lasheras, Krisztina J. Kovács, M. Victoria Milanés, Cristina Núñez. Hypothalamic Orexin-A Neurons Are Involved in the Response of the Brain Stress System to Morphine Withdrawal. PLoS ONE: Research Article, published 09 May 2012 10.1371/journal.pone.0036871

...guinea pig anti-DYN antibody (1∶2000, Neuromics, Edina, MN)...

B.A.S. Reyes, A.F. Carvalho, K. Vakharia. and E.J. Van Bockstaele. Amygdalar peptidergic circuits regulating noradrenergic locus coeruleus neurons: Linking limbic and arousal centers. doi:10.1016/j.expneurol.2011.04.001.

...cocktail containing guinea pig antibody directed against prodynorphin (ppDYN; Neuromics, Inc.)...

Wulf Haubensak, Prabhat S. Kunwar, Haijiang Cai1, Stephane Ciocchi, Nicholas R. Wall, Ravikumar Ponnusamy, Jonathan Biag, Hong-Wei Dong, Karl Deisseroth, Edward M. Callaway, Michael S. Fanselow, Andreas Lüthi & David J. Anderson. Genetic dissection of an amygdala microcircuit that gates conditioned fear. Nature 468, 270-276 (11 November 2010) | doi:10.1038/nature09553.

...guinea-pig anti-dynorphin (GP10110; Neuromics)...

Melissa L. Perreault, Ahmed Hasbi, Mohammed Alijaniaram, Theresa Fan, George Varghese, Paul J. Fletcher, Philip Seeman, Brian F. O'Dowd, and Susan R. George. The Dopamine D1-D2 Receptor Heteromer Localizes in Dynorphin/Enkephalin Neurons: INCREASED HIGH AFFINITY STATE FOLLOWING AMPHETAMINE AND IN SCHIZOPHRENIA. J. Biol. Chem., Nov 2010; 285: 36625 - 36634.

...DYN (guinea pig) from Neuromics...

Cristina Núñez, Fátima Martín, Anna Földes, M. Luisa Laorden, Krisztina J. Kovács, M. Victoria Milanés. Induction of FosB/ΔFosB in the brain stress system-related structures during morphine dependence and withdrawal. Journal of Neurochemistry. DOI: 10.1111/j.1471-4159.2010.06765.x

...rat pro-DYN; # GP10110 was purchased from Neuromics (Edina, MN)...

N. Maolood and B. Meister. Nociceptin/Orphanin FQ Peptide in Hypothalamic Neurones Associated with the Control of Feeding Behaviour. Journal of Neuroendocrinology. Volume 22 Issue 2, Pages 75 - 82. Published Online: 15 Dec 2009.

...1 : 4000; GP10110; Neuromics... 

Chris Lough, Tracey Young, Renee Parker, Shannon Wittenauer and Michelle Vincler. Increased spinal dynorphin contributes to chronic nicotine-induced mechanical hypersensitivity in the rat.  doi:10.1016/j.neulet.2007.06.002 

...anti-prodynorphin (1:4000, Neuromics, Edina, MN)...

Kyle G. Halvorson, BA, Molly A. Sevcik, BA, Joseph R. Ghilardi, BS, BA, Lucy J. Sullivan, BA, Nathan J. Koewler, BS, Frieder Bauss, PhD, and Patrick W. Mantyh, PhD. Intravenous Ibandronate Rapidly Reduces Pain, Neurochemical Indices of Central Sensitization, Tumor Burden, and Skeletal Destruction in a Mouse Model of Bone Cancer. Published online 2008 April 14. doi: 10.1016/j.jpainsymman.2007.10.005 

Xiaying Zhua, Michelle A. Vincler, Renee Parker and James C. Eisenach. Spinal cord dynorphin expression increases, but does not drive microglial prostaglandin production or mechanical hypersensitivity after incisional surgery in rats. doi:10.1016/j.pain.2006.04.027

...anti-prodynorphin (1:4000, Neuromics, Edina, MN)...

Gardell LR, Wang R, Ehrenfels C, Ossipov MH, Rossimando AJ, Miller S, Buckley C, Cai AK, Tse A, Foley SF, Gong B, Walua L, Carmillo P, Worley D, Huang C, Engbeer T, Pepinsky B, Cate RL, Vanderah TW, Lai J, Sah DWY, Porreca F (2003). Multiple actions of systemic artemin in experimental neuropathy. Nature Med.  9: 1383-1389.

Sabino MC, Ghilardi JR, Jongen JLM, Keyser CP, Luger NM, Mach DB, Peters CM, Rogers SD, Schwei MJ, de Felipe C, Mantyh PW (2002).  Simultaneous Reduction in Cancer Pain, Bone Destruction, and Tumor Growth by Selective Inhibition of Cyclooxygenase-2. Cancer Resear. 62:7343-7349.

...Mice were sacrificed at day 10 or 14 post-tumor injection and the tissues were processed for immunohistochemical analysis of spinal cord and femora as previously described.14, 17, 27, 28 Briefly, mice received a normally non-noxious mechanical stimulation of the injected femur 90 minutes prior to euthanasia for induction of c-Fos expression.27, 29 Following this manipulation, mice were euthanized with CO2 and perfused intracardially with 12ml 0.1 M phosphate buffered saline (PBS) followed by 25 ml 4% formaldehyde/12.5% picric acid solution.

Spinal cord segments (L2–L4) were removed, post-fixed in the perfusion fixative overnight and cryoprotected in 30% sucrose at 4° C for 24 hours. Serial frozen spinal cord sections, 60 µm thick, were cut on a sliding microtome, collected in PBS, and processed as free floating sections.

Following sectioning, spinal cord sections were briefly rinsed in PBS and then incubated in blocking solution at 22° C (3% normal donkey serum (NDS) 0.3% Triton X-100 in PBS) for one hr followed by incubation overnight at 22° C in the primary antibody. Spinal cord sections were immunostained for c-Fos protein (1:2,000, Oncogene Research, San Diego, California, USA) and prodynorphin (polyclonal guinea pig anti-prodynorphin, 1:1,000, Neuromics, Minneapolis, Minnesota, USA).

After incubation in primary antibody solutions, sections were rinsed in PBS three times for 10 minutes each and then incubated in the secondary antibody solution for 3 hours at 22° C. Secondary antibodies, conjugated to Cy3 or biotin (Jackson ImmunoResearch, West Grove, Pennsylvania, USA), were used at 1:600 or 1:500, respectively. In order to detect secondary antibodies conjugated to biotin, following secondary incubation, sections were rinsed in PBS and incubated in Cy3 conjugated streptavidin (1:4000; Jackson ImmunoResearch) for 45 minutes. To confirm specificity of the primary antibodies, controls included omission of the primary antibody or preabsorption with the corresponding synthetic peptide. Following immunostaining procedures, spinal cord sections were mounted onto gelatin-coated slides. All mounted sections were then dehydrated in a successive alcohol gradient scheme (70, 90, 100%), cleared in xylene and coverslipped with DPX (Fluka, Buchs, Switzerland)...